Clinically and Industrially Relevant Peanut Reference Materials to Improve Analysis of Food Allergens

24 Pages Posted: 29 Jun 2023

See all articles by Gill Holcombe

Gill Holcombe

LGC, Ltd.

Michael Walker

LGC, Ltd.

Malvinder Singh

LGC, Ltd.

Stephen L. R. Ellison

LGC, Ltd.

Simon Cowen

LGC, Ltd.

Joanna Topping

LGC, Ltd.

Adrian Rogers

Bio-Check (UK), Ltd.; Romer Labs UK, Ltd.

Phil E. Johnson

The University of Manchester - The Manchester Institute of Biotechnology

Rosemary Adaba

The University of Manchester - The Manchester Institute of Biotechnology

Chiara Nitride

University of Naples Federico II

Anuradha Balasundaram

The University of Manchester - The Manchester Institute of Biotechnology

E. N. Clare Mills

The University of Manchester - The Manchester Institute of Biotechnology

Abstract

Food allergy requires effective food allergen analysis to support risk analysis and risk management. There are acknowledged shortcomings in allergen analysis which could be better addressed by appropriate reference materials (RM). An RM for peanut has been developed based on a matrix and a partially defatted light roasted peanut flour used for the EuroPrevall clinical threshold studies. The RM has been prepared as a matrix devoid of peanut and the matrix spiked with peanut flour at the clinically relevant concentration of 10 mg kg-1 peanut protein. The partially defatted light roasted peanut flour is also available as a RM. Homogeneity and stability have been characterised and statistically assessed as fit-for-purpose. Establishing the homogeneity of the incurred RM was challenging owing to the dispersion of the data obtained using ELISA. The material is available and presents a model for food allergen RM production, characterisation and curation.

Keywords: Food Allergen, Reference Material, Peanut protein

Suggested Citation

Holcombe, Gill and Walker, Michael and Singh, Malvinder and Ellison, Stephen L. R. and Cowen, Simon and Topping, Joanna and Rogers, Adrian and Johnson, Phil E. and Adaba, Rosemary and Nitride, Chiara and Balasundaram, Anuradha and Mills, E. N. Clare, Clinically and Industrially Relevant Peanut Reference Materials to Improve Analysis of Food Allergens. Available at SSRN: https://ssrn.com/abstract=4481493 or http://dx.doi.org/10.2139/ssrn.4481493

Gill Holcombe

LGC, Ltd. ( email )

Malvinder Singh

LGC, Ltd. ( email )

Simon Cowen

LGC, Ltd. ( email )

Joanna Topping

LGC, Ltd. ( email )

Adrian Rogers

Bio-Check (UK), Ltd. ( email )

Romer Labs UK, Ltd. ( email )

Phil E. Johnson

The University of Manchester - The Manchester Institute of Biotechnology ( email )

Rosemary Adaba

The University of Manchester - The Manchester Institute of Biotechnology ( email )

Chiara Nitride

University of Naples Federico II ( email )

Naples
Italy

Anuradha Balasundaram

The University of Manchester - The Manchester Institute of Biotechnology ( email )

E. N. Clare Mills

The University of Manchester - The Manchester Institute of Biotechnology ( email )

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