Overexpression of Atbzip69 Gene in Transgenic Wheat Confers Tolerance to Nitrogen Stress and Increases Grain Yield

28 Pages Posted: 16 Jun 2023

See all articles by Jiji Yan

Jiji Yan

Shanxi Normal University

Daoping Wang

affiliation not provided to SSRN

Shuyu Kang

Shanxi Normal University

Zhang He

Harbin Normal University

Xin Li

affiliation not provided to SSRN

Huawei Shi

affiliation not provided to SSRN

Wensi Tang

affiliation not provided to SSRN

Kai Chen

affiliation not provided to SSRN

Yongbin Zhou

affiliation not provided to SSRN

Zhaoshi Xu

affiliation not provided to SSRN

Jun Chen

affiliation not provided to SSRN

Youzhi Ma

affiliation not provided to SSRN

Ming Chen

affiliation not provided to SSRN

Chunhua Pang

Shanxi Normal University

Abstract

Nitrogen is an important nutrient for the growth and yield of wheat. However, in practical production, wheat suffers from nitrogen stress. In this study, we generated wheat plants with improved low nitrogen (LN) tolerance by introducing AtbZIP69, a gene encoding a basic leucine zipper domain transcription factor, into the wheat variety Shi 4056. We observed that AtbZIP69 expression in transgenic wheat was induced by LN and drought stress. AtbZIP69 localised in the nucleus and activated transcription. A greenhouse trial revealed that, compared to wild type (WT) wheat, the AtbZIP69 transgenic wheat showed significantly increased drought and LN stress tolerance. A two-year field trial demonstrated that the yield and number of spikes of transgenic wheat were significantly higher than those of WT wheat under LN conditions. By investigating nitrogen use-related traits, we discovered that overexpression of AtbZIP69 altered nitrogen distribution by allocating more nitrogen to grains under LN conditions. In addition, the expression of nitrogen transporter protein genes (such as TaNRT2.1, TaNRT2.5, and TaNR1) was higher in AtbZIP69 transgenic wheat than in WT wheat under LN conditions. Overall, AtbZIP69 overexpression increased nitrogen content of the transgenic line grains under LN conditions by regulating the expression of nitrogen-related transporter protein genes, potentially leading to increased grain yield. As a result, a new candidate gene for improving LN stress tolerance in the field was identified using heterologous expression analysis of wheat.

Keywords: bZIP transcription factors, low nitrogen stress, grain yield, transgenic wheat

Suggested Citation

Yan, Jiji and Wang, Daoping and Kang, Shuyu and He, Zhang and Li, Xin and Shi, Huawei and Tang, Wensi and Chen, Kai and Zhou, Yongbin and Xu, Zhaoshi and Chen, Jun and Ma, Youzhi and Chen, Ming and Pang, Chunhua, Overexpression of Atbzip69 Gene in Transgenic Wheat Confers Tolerance to Nitrogen Stress and Increases Grain Yield. Available at SSRN: https://ssrn.com/abstract=4481815 or http://dx.doi.org/10.2139/ssrn.4481815

Jiji Yan

Shanxi Normal University ( email )

Lifen
China

Daoping Wang

affiliation not provided to SSRN ( email )

No Address Available

Shuyu Kang

Shanxi Normal University ( email )

Lifen
China

Zhang He

Harbin Normal University ( email )

Harbin
China

Xin Li

affiliation not provided to SSRN ( email )

No Address Available

Huawei Shi

affiliation not provided to SSRN ( email )

No Address Available

Wensi Tang

affiliation not provided to SSRN ( email )

No Address Available

Kai Chen

affiliation not provided to SSRN ( email )

No Address Available

Yongbin Zhou

affiliation not provided to SSRN ( email )

No Address Available

Zhaoshi Xu

affiliation not provided to SSRN ( email )

No Address Available

Jun Chen

affiliation not provided to SSRN ( email )

No Address Available

Youzhi Ma

affiliation not provided to SSRN ( email )

No Address Available

Ming Chen

affiliation not provided to SSRN ( email )

No Address Available

Chunhua Pang (Contact Author)

Shanxi Normal University ( email )

Lifen
China

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