Dendrobium Officinale Regulates Bile Acid Synthesis to Improve Acute Alcoholic Liver Injury in Mice

Abstract

Alcoholic liver disease (ALD) is the most common type of chronic liver disease worldwide, which is linked to bile acid (BA) metabolism dysregulation. Large population studies showed that dendrobium officinale (D. officinale) could ameliorate liver lipid accumulation, however, the underlying mechanism of D. officinale altered BA metabolism in hepatic is still unclear. In this study, the protective effects of D. officinale aqueous extract (DOAE) on acute alcoholic liver injury (AALI) in mice were evaluated by determining the serum biochemical indicators and liver pathology, and the mechanism was investigated by analyzing the alteration of BA through liquid chromatography-mass spectrometry (LC-MS) and RT-PCR. The results showed that DOAE could protect AALI by inhibiting the alcohol-induced elevation of serum AST and ALT levels and dyslipidemia. Moreover, DOAE could change the composition and content of hepatic BAs in mice, especially regulate hepatic BAs synthesis by activating the hepatic FXR-SHP signaling axis with subsequently decreased the expression of cholesterol 7 α-hydroxylase (CYP7A1) which mediated the synthesis of CDCA. Therefore, BA metabolism may be the potential target of the protective effect of DOAE.

Note:
Funding declaration: The study was supported by Guizhou Engineering Research Center of Industrial Key-technology for Dendrobium Nobile (QJJ[2022]048), the Ministry of Science and Technology of China (2021YFD1601001), Guizhou Provincial Education Department(QJJ[2022]006), the Department of Science and Technology of Guizhou Province (QKHZC[2021]420)

Conflict of Interests: The authors declare that they have no conflicts of interest

Ethical Approval: All procedures involving the use of laboratory animals were to the requirements of the Animal Experiment Ethics Committee of Zunyi Medical University.

Keywords: Dendrobium officinale, Acute alcoholic liver injury, Bile acids, LC-MS