Enhancement of Biocatalytic Activity in Enzymatic Membrane Reactors: Controlled Modification with Novel Pah/Pda Composites as a Tool to Optimize Reactor Performance

25 Pages Posted: 18 Jul 2023

See all articles by Manuel Pinelo

Manuel Pinelo

Technical University of Denmark

Andrei Popkov

Technical University of Denmark

Ziran Su

Technical University of Denmark

Magdalena Malankowska

Technical University of Denmark

Abstract

Enzymatic membrane (EM[1]) is a tool allowing to simultaneously upgrade enzymatic catalysis and membrane separation processes. However, their commercial use is often restricted by a trade-off between catalytic and mass transfer properties of the system. Herein, we report the design of a new enzymatic membrane reactor (EMR) with the optimum balance between biocatalytic activity, water permeability, and enzyme loading; where we used co-deposited polyallylamine/polydopamine (PAH/PDA) membrane coatings as a tool to control the overall reactor performance. First, we demonstrate that tailoring the PAH/PDA coating chemistry can be more effective for promotion of total biocatalytic activity of EM with immobilized Alcohol Dehydrogenase (ADH) than increasing the amount of immobilized enzyme on a membrane support. Then, we show how the PAH/PDA ratio (0, 1:10, 1:2, 5:2), modification time (0.25, 1, 4, 16 h) and ionic strength (0.1 – 1.0 M NaCl) affect morphology of the membrane coating and alter the mechanisms of ADH-support interactions. Lastly, we illustrate the versatility of the PAH/PDA-coated membranes for application in other enzymatic systems on the example of Glycerol Dehydrogenase (GDH) and evaluate the performance of two EMRs as a function of applied pressure (1-3 bar). The final values of biocatalytic activities 25.4±4.0 and 3.5±0.3 mU/cm2 (which correspond to 140±22 and 67±5% immobilization efficiencies) for immobilized ADH and GDH, respectively, demonstrate that PAH/PDA-coated membranes not only can be used for simultaneous reaction and recovery of the biocatalyst, but also for enhancement of enzymatic activity -  due to opportunity of reaction rate control in the membrane reactor.

Keywords: biocatalytic membrane, polyelectrolyte coating, polydopamine co-deposition, enzyme immobilization, membrane reactor

Suggested Citation

Pinelo, Manuel and Popkov, Andrei and Su, Ziran and Malankowska, Magdalena, Enhancement of Biocatalytic Activity in Enzymatic Membrane Reactors: Controlled Modification with Novel Pah/Pda Composites as a Tool to Optimize Reactor Performance. Available at SSRN: https://ssrn.com/abstract=4513999 or http://dx.doi.org/10.2139/ssrn.4513999

Manuel Pinelo (Contact Author)

Technical University of Denmark ( email )

Anker Engelunds Vej 1
Building 101A
Lyngby, 2800
Denmark

Andrei Popkov

Technical University of Denmark ( email )

Anker Engelunds Vej 1
Building 101A
Lyngby, 2800
Denmark

Ziran Su

Technical University of Denmark ( email )

Anker Engelunds Vej 1
Building 101A
Lyngby, 2800
Denmark

Magdalena Malankowska

Technical University of Denmark ( email )

Anker Engelunds Vej 1
Building 101A
Lyngby, 2800
Denmark

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