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Contribution of Sugar Transporters to Spatially Organized Colonization by Microbiota Along the Longitudinal Root Axis of Arabidopsis

59 Pages Posted: 25 Jul 2023 Publication Status: Published

See all articles by Eliza Loo

Eliza Loo

Heinrich Heine University Dusseldorf - Institute for Molecular Physiology

Paloma Durán

Max Planck Institute for Plant Breeding Research - Department of Plant Microbe Interactions

Tin Yau Pang

Heinrich Heine University Dusseldorf - Institute for Computer Science

Philipp Westhoff

Heinrich Heine University Dusseldorf - Plant Metabolism and Metabolomics Laboratory

Chen Deng

Heinrich Heine University Dusseldorf - Institute for Molecular Physiology

Ruben Garrido-Oter

Max Planck Institute for Plant Breeding Research - Department of Plant Microbe Interactions

Wolf Frommer

Heinrich Heine University Dusseldorf - Institute for Molecular Physiology

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Abstract

Plant roots are functionally heterogeneous in cellular architecture, transcriptome profile, metabolic state, and microbial immunity. We hypothesized that axial differentiation also impacts spatial colonization by root microbiota along the root axis. We developed two growth systems, ArtSoil and CD-Rhizotrons, to grow and then dissect Arabidopsis thaliana roots into three segments. We identified distinct root and rhizosphere bacterial communities in the segments, supporting the hypothesis of microbiota differentiation along the axis. Root metabolite profiling of each segment revealed differential metabolite enrichment and specificity. Bioinformatic analyses and GUS histochemistry indicated sugar and/or microbe-induced accumulation of SWEET2, 4, and 12 sugar uniporters. Profiling of root segments from sweet mutants showed altered endospheric root microbiota spatial organization. This work reveals the interdependency between root physiology and microbial colonization, and the contribution of SWEETs to shaping local endospheric root microbiota.

Keywords: spatial microbiota organization, SWEET sugar transporters, spatial metabolite accumulation, root microbe-metabolite interactions

Suggested Citation

Loo, Eliza and Durán, Paloma and Pang, Tin Yau and Westhoff, Philipp and Deng, Chen and Garrido-Oter, Ruben and Frommer, Wolf, Contribution of Sugar Transporters to Spatially Organized Colonization by Microbiota Along the Longitudinal Root Axis of Arabidopsis. Available at SSRN: https://ssrn.com/abstract=4514131 or http://dx.doi.org/10.2139/ssrn.4514131
This version of the paper has not been formally peer reviewed.

Eliza Loo (Contact Author)

Heinrich Heine University Dusseldorf - Institute for Molecular Physiology ( email )

Paloma Durán

Max Planck Institute for Plant Breeding Research - Department of Plant Microbe Interactions ( email )

Tin Yau Pang

Heinrich Heine University Dusseldorf - Institute for Computer Science ( email )

Philipp Westhoff

Heinrich Heine University Dusseldorf - Plant Metabolism and Metabolomics Laboratory ( email )

Chen Deng

Heinrich Heine University Dusseldorf - Institute for Molecular Physiology ( email )

Ruben Garrido-Oter

Max Planck Institute for Plant Breeding Research - Department of Plant Microbe Interactions ( email )

Wolf Frommer

Heinrich Heine University Dusseldorf - Institute for Molecular Physiology ( email )

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