A Novel Fluorescein Ligand Fluorescent Probe for Multi-Ion Detection

Using fluorescein and thiophene hydrazine, a new multi-ion detection fluorescent probe called YS was created in this study. It can detect Al3+ and Cu2+ by the fluorescence on and off phenomena, respectively. The binding ratio of YS to both Al3+ and Cu2+ was calculated by Job's plot as 1:1, and the binding constants of YS to Al3+ and Cu2+ were calculated by Benesi-Hildebrand method as 1.38×105 M-1 and 2.09×104 M-1, respectively. Finally, by examining the data from the fluorescence gradient concentration test, It can be observed that the fluorescence probe YS has high sensitivity and selectivity for both Al3+and Cu2+, with a minimum detection limit of at 12.65nM and 4.732nM, both of which are lower than the WHO standards for the corresponding ions in drinking water. The convenience detection test demonstrated that YS can achieve in situ rapid detection of Al3+ and the results are easy to observe and distinguish.

Keywords: Fluorescein；Thiophene hydrazine；Multi-ion detection；PET

Suggested Citation: Suggested Citation

Wang, Jundong and Pei, Yanglan and Hu, Kun and Han, Lu and Mo, Lixin and Qi, Yuansheng and Li, Luhai and Wei, Yen, A Novel Fluorescein Ligand Fluorescent Probe for Multi-Ion Detection. Available at SSRN: https://ssrn.com/abstract=4522518 or http://dx.doi.org/10.2139/ssrn.4522518