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Detection of Ultraweak Photon Emissions from Mouse Embryos with Implications for Assisted Reproduction

20 Pages Posted: 8 Aug 2023

See all articles by Jozsef Bodis

Jozsef Bodis

University of Pécs

Zoltan Bognar

University of Pécs

Timea Csabai

University of Pécs

Jozsef Berke

University of Pécs

Istvan Gulyas

University of Pécs

Peter Mauchart

University of Pécs

Hans-Rudolf Tinneberg

University of Giessen

Balint Farkas

University of Pécs

Akos Varnagy

University of Pécs - Hungarian National Laboratory on Reproduction

Kalman Kovacs

University of Pécs - Hungarian National Laboratory on Reproduction

More...

Multiple version iconThere are 2 versions of this paper

Abstract

Background: In the past four decades, in vitro fertilization (IVF) has benefited from substantial advancements and become a routine medical procedure. Embryo development can be moderated with time-lapse systems, but such systems use visible light that can harm cells. Living cells have spontaneous ultraweak photon emissions (UPEs) that are generated by metabolic reactions and influenced by physiological conditions.

Methods: Embryo-emitted photons were detected with a custom in-house ORCA-Quest CMOS camera and microscope incubator system. Images were taken in the dark. Negative control measures were taken for an empty vessel and a vessel with only oil and media. Optimal data were collected with all software filters off.

Findings: Reference measurements showed only negligible differences between empty and incubation-medium filled samples. When four-cell embryos were removed from their culture incubators for examination in laboratory air, light, and temperature conditions, degenerated two-cell stage embryos were observed to have lower UPE levels than cleaving embryos. Fresh embryos had significantly greater UPE levels than previously frozen and then thawed embryos.

Interpretation: UPE detection in mouse embryos can form a realistic basis for the development of a photon emission embryo control system.

Funding: The current study was funded by the European Union (RRF-2.3.1-21-2022-00012 National Laboratory on Human Reproduction).

Declaration of Interest: None.

Ethical Approval: The study was conducted according to the guidelines of the Declaration of Helsinki and approved by the Regional and Local Research Ethics Committee of the University of Pécs, Pécs, Hungary (#PTE KK 7072-2018). All methods were carried out in accordance with relevant guidelines and regulations of the Animal Health Committee of Baranya.

Keywords: photon emission, mice embryo, spectral fingerprint, IVF, EW-SFD

Suggested Citation

Bodis, Jozsef and Bognar, Zoltan and Csabai, Timea and Berke, Jozsef and Gulyas, Istvan and Mauchart, Peter and Tinneberg, Hans-Rudolf and Farkas, Balint and Varnagy, Akos and Kovacs, Kalman, Detection of Ultraweak Photon Emissions from Mouse Embryos with Implications for Assisted Reproduction. Available at SSRN: https://ssrn.com/abstract=4531629 or http://dx.doi.org/10.2139/ssrn.4531629

Jozsef Bodis

University of Pécs ( email )

Rákóczi út 80
Pécs, 7622
Hungary

Zoltan Bognar

University of Pécs ( email )

Rákóczi út 80
Pécs, 7622
Hungary

Timea Csabai

University of Pécs ( email )

Rákóczi út 80
Pécs, 7622
Hungary

Jozsef Berke

University of Pécs ( email )

Rákóczi út 80
Pécs, 7622
Hungary

Istvan Gulyas

University of Pécs ( email )

Rákóczi út 80
Pécs, 7622
Hungary

Peter Mauchart

University of Pécs ( email )

Rákóczi út 80
Pécs, 7622
Hungary

Hans-Rudolf Tinneberg

University of Giessen ( email )

Germany

Balint Farkas (Contact Author)

University of Pécs ( email )

Akos Varnagy

University of Pécs - Hungarian National Laboratory on Reproduction ( email )

Kalman Kovacs

University of Pécs - Hungarian National Laboratory on Reproduction ( email )