Preprints with The Lancet is part of SSRN´s First Look, a place where journals identify content of interest prior to publication. Authors have opted in at submission to The Lancet family of journals to post their preprints on Preprints with The Lancet. The usual SSRN checks and a Lancet-specific check for appropriateness and transparency have been applied. Preprints available here are not Lancet publications or necessarily under review with a Lancet journal. These preprints are early stage research papers that have not been peer-reviewed. The findings should not be used for clinical or public health decision making and should not be presented to a lay audience without highlighting that they are preliminary and have not been peer-reviewed. For more information on this collaboration, see the comments published in The Lancet about the trial period, and our decision to make this a permanent offering, or visit The Lancet´s FAQ page, and for any feedback please contact preprints@lancet.com.
Detection of Ultraweak Photon Emissions from Mouse Embryos with Implications for Assisted Reproduction
20 Pages Posted: 8 Aug 2023
More...There are 2 versions of this paper
Detection of Ultraweak Photon Emissions from Mouse Embryos with Implications for Assisted Reproduction
Detection of Ultraweak Photon Emissions from Mouse Embryos with Implications for Assisted Reproduction
Abstract
Background: In the past four decades, in vitro fertilization (IVF) has benefited from substantial advancements and become a routine medical procedure. Embryo development can be moderated with time-lapse systems, but such systems use visible light that can harm cells. Living cells have spontaneous ultraweak photon emissions (UPEs) that are generated by metabolic reactions and influenced by physiological conditions.
Methods: Embryo-emitted photons were detected with a custom in-house ORCA-Quest CMOS camera and microscope incubator system. Images were taken in the dark. Negative control measures were taken for an empty vessel and a vessel with only oil and media. Optimal data were collected with all software filters off.
Findings: Reference measurements showed only negligible differences between empty and incubation-medium filled samples. When four-cell embryos were removed from their culture incubators for examination in laboratory air, light, and temperature conditions, degenerated two-cell stage embryos were observed to have lower UPE levels than cleaving embryos. Fresh embryos had significantly greater UPE levels than previously frozen and then thawed embryos.
Interpretation: UPE detection in mouse embryos can form a realistic basis for the development of a photon emission embryo control system.
Funding: The current study was funded by the European Union (RRF-2.3.1-21-2022-00012 National Laboratory on Human Reproduction).
Declaration of Interest: None.
Ethical Approval: The study was conducted according to the guidelines of the Declaration of Helsinki and approved by the Regional and Local Research Ethics Committee of the University of Pécs, Pécs, Hungary (#PTE KK 7072-2018). All methods were carried out in accordance with relevant guidelines and regulations of the Animal Health Committee of Baranya.
Keywords: photon emission, mice embryo, spectral fingerprint, IVF, EW-SFD
Suggested Citation: Suggested Citation