Comparison of the Arsenic Protective Effects of Four Nanomaterials on Pakchoi in an Alkaline Soil

Abstract

Accurately applying engineering nanoparticles (NPs) in farmland stress management is important for sustainable agriculture and food safety. The protective effects of four engineered NPs (SiO2, CeO2, ZnO, and S) on pakchoi under arsenic (As) stress were investigated using pot experiments. The results showed that CeO2, SiO2, and S NPs resulted in biomass reduction, while ZnO NPs (100 and 500 mg kg-1) significantly increased shoot height. Although 500 mg kg-1 S NPs rapidly dissolved to release SO42-, reducing soil pH and pore water As content and further reducing shoot As content by 21.6%, the growth phenotype was inferior to that obtained with 100 mg kg-1 ZnO NPs, probably due to acid damage. The addition of 100 mg kg-1 ZnO NPs not only significantly reduced the total As content in pakchoi by 23.9% compared to the As-alone treatment but also enhanced plant antioxidative activity by increasing superoxide dismutase and peroxidase activities and decreasing malondialdehyde content. ZnO NPs in soil might inhibit As uptake by roots by increasing the dissolved organic carbon (DOC) in pore water. According to the DLVO theory, ZnO NPs were the most effective in preventing As in pore water from entering plant roots due to their smaller hydrated particle size. Redundancy analysis further confirmed that DOC and SO42- were the primary factors controlling plant As uptake under the ZnO NP and S NP treatments, respectively. Our findings provide fundamental information for the safe and sustainable application of NP-conjugated agrochemicals.

Keywords: pakchoi, nanoparticles, arsenic control, soil, redundancy analysis.