Trifloxystrobin Induced Developmental Toxicity by Disturbing the ABC Transporters, Carbohydrate and Lipid Metabolism in Adult Zebrafish

Abstract

Trifloxystrobin (TR) caused multiplex toxicity on aquatic organisms, but few studies have paid close attention to its chronic toxicity at environmental concentrations. In present study, histopathology, metabolomics and transcriptomics was performed to investigate the toxic effects and biological responses on adult zebrafish after exposure to 0.1, 1 and 10 μg/L TR for 21 d. Results demonstrated long-term exposure of TR affected zebrafish liver, ovary and heart development. Metabolomics revealed 0.1, 1 and 10 μg/L TR simultaneously decreased the carbohydrates enriched in glucose metabolism and ABC transporters pathways, such as glycogen, lactose, lactulose, maltose, maltotriose, D-trehalose, while 1 μg/L and 10 μg/L TR significantly increased many metabolites in glycerophospholipid and sphingolipid metabolism. Transcriptomics showed TR activated the transcription of the key genes involved in ABC transporters, glucose and lipid metabolism in zebrafish liver. Results demonstrated high concentration of TR strongly affected the DNA repair system, while low level of TR had pronounced effects on cardiomyocytes and oocyte regulation pathways at transcriptional levels, indicating low dose effects caused by TR deserved more attention. The results indicated the growth damage caused by TR might be ascribed to the disturbance of carbohydrates and lipid metabolism mediating by Abcb4, Abcb5 and Abcb11 ABC transporters.

Keywords: Trifloxystrobin, Metabolomics, transcriptomic, Zebrafish, Carbohydrate and lipid metabolism