Revolutionizing Ventilator-Associated Pneumonia Diagnosis: Electrochemical Sensors for Rapid and Sensitive Detection of Pyocyanin

23 Pages Posted: 2 Nov 2023

See all articles by Mohammad Noorizadeh

Mohammad Noorizadeh

Qatar University

Tamim M. Al-Hasan

Qatar University

Mizaj S. Sha

Qatar University

Faycal Bensaali

Qatar University

Nader Meskin

Qatar University

Kishor Kumar Sadasivuni

Qatar University

Susu M. Zughaier

Qatar University

Mahmoud Elgamal

Qatar University

Ali Ait Hssain

Hamad Medical Corporation - Medical Intensive Care Unit

Abstract

Ventilator-associated pneumonia (VAP) is a severe condition in critically ill patients that requires timely diagnosis to improve patient outcomes. In this study, we developed an electrochemical sensor for pyocyanin detection using a TiO2-NiO-rGO catalyst and found that it could detect the concentration of pyocyanin required for mimicking the throat trachea’s environment (LOD: 0.2 µg/mL). We also investigated the use of DNA aptamer for detecting pyocyanin, a biomarker of Pseudomonas aeruginosa, a common causative agent of VAP. Our findings suggest that the TiO2-NiO-rGO catalyst without aptamer could provide a rapid and cost-effective diagnostic tool for VAP, and the electrochemical sensor has the potential to be made disposable for in vivo applications. Using the TiO2-NiO-rGO catalyst offers synergistic effects that enhance the overall electrochemical performance of the detection process, sensitivity, and selectivity. This work’s results demonstrate that electrochemical detection of pyocyanin using a TiO2-NiO-rGO catalyst holds great potential for various applications, such as clinical diagnostics and drug discovery. Further research and optimization of the catalyst composition, morphology, and surface modifications can contribute to advancing this pyocyanin electrochemical detection approach.

Keywords: Biosensors, Bio-Signal Processing, DNA Aptamer, Electrochemistry, Nosocomial infections, Ventilator Associated Pneumonia (VAP)

Suggested Citation

Noorizadeh, Mohammad and Al-Hasan, Tamim M. and Sha, Mizaj S. and Bensaali, Faycal and Meskin, Nader and Sadasivuni, Kishor Kumar and Zughaier, Susu M. and Elgamal, Mahmoud and Hssain, Ali Ait, Revolutionizing Ventilator-Associated Pneumonia Diagnosis: Electrochemical Sensors for Rapid and Sensitive Detection of Pyocyanin. Available at SSRN: https://ssrn.com/abstract=4615807 or http://dx.doi.org/10.2139/ssrn.4615807

Tamim M. Al-Hasan

Qatar University ( email )

Mizaj S. Sha

Qatar University ( email )

College of Law
Qatar University
Doha, 2713
Qatar

Faycal Bensaali

Qatar University ( email )

Nader Meskin

Qatar University ( email )

College of Law
Qatar University
Doha, 2713
Qatar

Kishor Kumar Sadasivuni

Qatar University ( email )

College of Law
Qatar University
Doha, 2713
Qatar

Susu M. Zughaier

Qatar University ( email )

Mahmoud Elgamal

Qatar University ( email )

Ali Ait Hssain

Hamad Medical Corporation - Medical Intensive Care Unit ( email )

Do you have a job opening that you would like to promote on SSRN?

Paper statistics

Downloads
17
Abstract Views
103
PlumX Metrics