Zno-S.Cerevisiae: An Effective Growth Promoter of Astragalus Memeranaceus and Nano-Antibacterial Agent Against Fusarium Oxysporum by Improving Trifolirhizin
47 Pages Posted: 14 Nov 2023
Abstract
Astragalus memeranaceus has been widely used in health, and root rot is a common disease in its cultivation, which is caused by Fusarium oxysporum. ZnO- Saccharomyces.cerevisiae was synthesized and its antibacterial activity against F.oxysporum was investigated in vitro and in vivo for the first time. It can directly inhibit pathogens by down-regulating physiological genes. In the plant experiment, the disease severity decreased in the ZnO-S.cerevisiae treated A.memeranaceus. Additionally, increased the content of chlorophyll and Astragaloside IV, and then improved the growth of A.memeranaceus seedlings. Furthermore, by analysis of muti-omic, enzymatic assay and molecular docking, the obtained ZnO-S.cerevisiae could activate the activities of the antioxidant enzymes and induce resistance genes in A.memeranaceus to secrete trifolirhizin, indicating higher resistance of plants under biotic stresses. The multifunctional ZnO-S.cerevisiae with superior antibacterial activity provides a promising alternative to the traditional antibacterial agents and has great potential in plant protection.
Keywords: ZnO-S.cerevisiae, Astragalus memeranaceus, Antibacterial, Fusarium oxysporum, Astragaloside IV
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