Multifunctional Photoelectrochemical Platform Based on Layer-by-Layer Assembly of Au/Bi2moo6/V2ctx for Simultaneous Detection and Inactivation of Salmonella Enteritidis

Abstract

A unique multifunctional photoelectrochemical(PEC) platform for the simultaneous detection and inactivation of Salmonella enteritidis(SE)was proposed. The photoactive material in the platform was a Bi2MoO6/V2CTx composite, and gold nanoparticles were modified to link the aptamer via Au-S bonds. The aptamer was to link the complementary DNA (cDNA) to the electrode surface via base complementarity. In the presence of SE, the aptamer attached to Salmonella enteritidis selectively and then the cDNA was released from the aptamer electrode, the photocurrent signal was amplified, allowing for quantitative detection of Salmonella enteritidis. The linear range of the method was 1.82×102 CFU/mL to 1.82×108 CFU/mL based on the specific recognition of Salmonella enteritidis by aptamer. The detection limit was 26 CFU/mL. Furthermore, due to its photoelectrocatalytic action, the PEC platform demonstrated strong antibacterial activity (99.99%). The new technique was created to detect and inactivate Salmonella enteritidis in a timely and sensitive manner.

Keywords: Photoelectrochemical platform, Salmonella enteritidis, Detection, Inactivation, Nanocomposites