Preliminary Investigation on the Potential Involvement of An Abc-Like Gene In Halomicronema Metazoicum (Cyanobacteria) Tolerance to Low Seawater Ph in an Ocean Acidification Scenarios

Abstract

Decreasing ocean surface pH, referred to as ocean acidification (OA), is among the major risks for marine ecosystems due to human-driven atmospheric pCO2 increase. Understanding the molecular mechanisms of adaptation enabling marine species to tolerate a lowered seawater pH could support predictions of consequences of future OA scenarios for marine life. This study examined whether the ATP-binding cassette (ABC)-like gene slr2019 confers tolerance to the marine cyanobacterium Halomicronema metazoicum to low seawater pH conditions (7.7, 7.2, 6.5) in short- and long-term exposures (7 and 30 d). Photosynthetic pigment content indicated that the species can tolerate all three lowered-pH conditions. At day 7, the slr2019 was up-regulated at pH 7.7 while no changes were observed at lower pH. After 30-d exposure, a significant decrease in slr2019 transcript levels was observed in all low-pH treatments. These first results indicate an effect of low pH on the examined transporter expression in H. metazoicum.

Keywords: Halomicronema metazoicum, Cyanobacteria, acid stress tolerance, ATP-binding cassette (ABC) membrane transporter proteins, ocean acidification (OA) scenarios, low pH