Ratiometric Luminescence Detection of H2o2 in Food Samples Using a Terbium Coordination Polymer Sensitized with 3-Carboxyphenylboronic Acid

Abstract

A ratiometric luminescent probe was fabricated using adenosine monophosphate (AMP) as a bridging ligand and 3-carboxyphenylboronic acid (3-CPBA) as the sensitizer and functional ligand that allowed the probe to recognize hydrogen peroxide (H2O2). The probe was labeled AMP-Tb/3-CPBA. Adding H2O2 caused the nonluminescent 3-CPBA to be converted into 3-hydroxybenzoic acid, which strongly luminesces at 401 nm. 3-CPBA is different from 4-carboxyphenylboronic acid (CPBA) reported in literature, as the reaction product between CPBA and H2O2 does not exhibit luminescent properties. This meant that adding H2O2 decreased the AMP-Tb/3-CPBA luminescence intensity at 544 nm and caused luminescence at 401 nm. The 401 and 544 nm luminescence intensity ratio (I401/I544) was strongly associated with the H2O2 concentration between 0.1 and 60.0 μM, and the detection limit was 0.23 μM. Dual emission reverse-change ratio luminescence sensing using the probe allowed environmental effects to be excluded and the assay to be very selective. We believe that the results pave the way for the development of new functionalized lanthanide coordination polymers for use in luminescence assays.

Keywords: H2O2, Ratiometric luminescence probes, 3-Carboxyphenylboronic acid, Terbium coordination polymer nanoparticles