Electrochemiluminescent Quenching of Luminol Dopped Zr-Mof for Application of Atp Detection Through Scavenging ROS by Gallic Acid Capped AU Nanoparticles

Abstract

In the present work, electrochemiluminescence (ECL) emissions of a Zr-based metal-organic framework coordinating with the amino of luminol (Zr-Lu-MOF) as highly efficient ECL luminophores was developed for highly effective sensing platform. In the presence of coreactant H2O2, Zr-Lu-MOF exhibited excellent and stable ECL behavior according to ECL measurements and cyclic voltammetry. Furthermore, ATP could be firmly assembled on Zr-Lu-MOF on the basis of the high affinity of Zr4+ toward phosphate groups. To sensitively detect ATP, gallic acid capped Au nanoparticles (GA@Au) was utilized to combine with ATP aptamer. It was found that GA@Au could be quenched the ECL behavior of luminol/H2O2 system and the quenching mechanism was ascribed to GA scavenging superoxide radical (O2•−) which was generated by electroredox of H2O2. Under optimal experiments, the ECL biosensor for detecting ATP with a wider linear range from 0.1 to 1000 nM and a detection limit of 0.035 nM (S/N = 3) was obtained. Thus, the proposed ECL biosensor exhibited good performance with high stability and acceptable fabrication reproducibility, which provided a valuable strategy for clinic diagnostics and therapeutics.

Keywords: reactive oxygen species, nano-antioxidant, luminol, electrochemiluminescence, Biosensor