An Optimized Whole-Body Corticosteroid Hormones Quantification Method by Lc-Ms/Ms for Assessing Stress Levels in European Glass Eels (Anguilla Anguilla)

29 Pages Posted: 1 Mar 2024

See all articles by Stellia Sebihi

Stellia Sebihi

affiliation not provided to SSRN

Monperrus Mathilde

Universite de Pau et des Pays de l’Adour

Pascale Coste

affiliation not provided to SSRN

Emmanuel Huchet

affiliation not provided to SSRN

Matthieu Lingrand

affiliation not provided to SSRN

Stéphane Glise

affiliation not provided to SSRN

Colin Bouchard

affiliation not provided to SSRN

Maren Ortiz-Zarragoitia

affiliation not provided to SSRN

Bolliet Valerie

affiliation not provided to SSRN

Abstract

The European eel (Anguilla anguilla) juvenile stage exhibits facultative estuarine migration; the causes of this behavior are yet unknown, but it may have an impact on the population's fate by altering the sex ratio. Recent studies have highlighted potential stress-related issues in glass eels settling in estuaries, however studying stress response in small organisms requires sensitive, accurate and precise analytical methods. The aims of the present study are (i) to develop a whole-body Liquid Chromatography-Mass Spectrometry (LC-MS/MS) method for the simultaneous determination of several stress hormones in low-body mass fish; (ii) to apply this method to glass eels to study their responses to acute stress (iii) to test the effect of anxiolytics (Diazepam) on these responses. Our results showed that enhanced LC-MS/MS analysis reduced detection limits and improved accuracy and precision for the quantification at the individual level. Following an acute stress, cortisol concentration significantly increased in glass eels and a 15 hours diazepam exposure significantly reduced cortisol levels highlighting a marked anxiolytic effect on this species.

Keywords: Glass eel, Stress response, Cortisol, Steroidal hormones, HPI axis, Hyphenated technique

Suggested Citation

Sebihi, Stellia and Mathilde, Monperrus and Coste, Pascale and Huchet, Emmanuel and Lingrand, Matthieu and Glise, Stéphane and Bouchard, Colin and Ortiz-Zarragoitia, Maren and Valerie, Bolliet, An Optimized Whole-Body Corticosteroid Hormones Quantification Method by Lc-Ms/Ms for Assessing Stress Levels in European Glass Eels (Anguilla Anguilla). Available at SSRN: https://ssrn.com/abstract=4745174 or http://dx.doi.org/10.2139/ssrn.4745174

Stellia Sebihi

affiliation not provided to SSRN ( email )

No Address Available

Monperrus Mathilde

Universite de Pau et des Pays de l’Adour ( email )

Pascale Coste

affiliation not provided to SSRN ( email )

No Address Available

Emmanuel Huchet

affiliation not provided to SSRN ( email )

No Address Available

Matthieu Lingrand

affiliation not provided to SSRN ( email )

No Address Available

Stéphane Glise

affiliation not provided to SSRN ( email )

No Address Available

Colin Bouchard

affiliation not provided to SSRN ( email )

No Address Available

Maren Ortiz-Zarragoitia

affiliation not provided to SSRN ( email )

No Address Available

Bolliet Valerie (Contact Author)

affiliation not provided to SSRN ( email )

No Address Available

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