Opting for Polyamines with Specific Structural Traits as a Strategy to Boost Performance of Enzymatic Membrane Reactors

21 Pages Posted: 19 Apr 2024

See all articles by Manuel Pinelo

Manuel Pinelo

Technical University of Denmark

Magdalena Malankowska

Technical University of Denmark

Ziran Su

Technical University of Denmark

Markus Simon De Martini

Technical University of Denmark

Shantanu Singh

Technical University of Denmark

Andrei Popkov

Technical University of Denmark

Abstract

Enzymatic membrane reactor (EMR) is a type of continuous-flow bioreactor offering easy separation and reuse of biocatalyst while giving opportunities to improve its performance. However, simultaneous enhancement of activity and stability of enzyme while retaining high membrane permeability poses a challenge for a single reactor. Herein, we propose a new method to optimize EMR system - by employing cationic polyelectrolytes with selected molecular weight, backbone, and amino group type for modification of membrane surface; after we have evaluated the effect of polyamine chemistry and membrane properties on each aspect of EMR performance. Polydopamine (PDA)-assisted co-deposition of polyamines (polyethyleneimine (PEI), PEI-graft-poly(ethylene glycol) (PEI-g-PEG), poly(allylamine hydrochloride) (PAH), and poly(diallyldimethylammonium chloride) (PDADMAC)) was used as the modification method; the membranes were characterized by water permeability, water contact angle (WCA), zeta potential (ZP), FTIR spectra, and SEM/EDX; and EMRs were assessed by biocatalytic activity at various pH, flux, immobilization yield and efficiency (activity recovery), enzyme leakage, pH/storage and thermal stability, and reusability. In this work, EMRs with immobilized alcohol dehydrogenase (ADH) showed activities up to 8.9 mU/cm2, retained up to 86% of initial activity in three conversion cycles without any reduction in flux, and allowed to increase non-optimal pH activity (pH 10) by up to 81% and improve storage stability by up to 53% compared to the free enzyme. We discovered statistically significant (p < 0.01) relationships between: (1) polyamine backbone type (linear vs branched) and membrane permeability; (2) membrane isoelectric point and immobilization yield; and (3) membrane WCA and immobilization efficiency. To our knowledge, this is the first time  the effect of polyelectrolyte chemistry on performance of immobilized enzyme is studied in a systematic manner.

Keywords: membrane reactor, enzyme immobilization, polyelectrolyte, polydopamine, membrane modification, biocatalysis

Suggested Citation

Pinelo, Manuel and Malankowska, Magdalena and Su, Ziran and De Martini, Markus Simon and Singh, Shantanu and Popkov, Andrei, Opting for Polyamines with Specific Structural Traits as a Strategy to Boost Performance of Enzymatic Membrane Reactors. Available at SSRN: https://ssrn.com/abstract=4801070 or http://dx.doi.org/10.2139/ssrn.4801070

Manuel Pinelo (Contact Author)

Technical University of Denmark ( email )

Anker Engelunds Vej 1
Building 101A
Lyngby, 2800
Denmark

Magdalena Malankowska

Technical University of Denmark ( email )

Anker Engelunds Vej 1
Building 101A
Lyngby, 2800
Denmark

Ziran Su

Technical University of Denmark ( email )

Anker Engelunds Vej 1
Building 101A
Lyngby, 2800
Denmark

Markus Simon De Martini

Technical University of Denmark ( email )

Anker Engelunds Vej 1
Building 101A
Lyngby, 2800
Denmark

Shantanu Singh

Technical University of Denmark ( email )

Anker Engelunds Vej 1
Building 101A
Lyngby, 2800
Denmark

Andrei Popkov

Technical University of Denmark ( email )

Anker Engelunds Vej 1
Building 101A
Lyngby, 2800
Denmark

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