A Mechanical Biosensor Based on Magnetic-Mediated Sensitization for 5-Ht Detection

Abstract

The real-time detection of neurotransmitters had attracted more attention due to the potential to provide vital information for pathology research, disease diagnosis, and clinical efficacy evaluation. Here, a magnetic-mediated biosensor was developed to improve sensitivity for detection of serotonin (5-HT). The PDMS membrane doped with magnetic particles serves as a conversion element, enhancing the deformation of the thin film caused by surface stress under the action of a magnetic field and amplifying the resistance response signal of the biosensor. The 5-HT detection associated with neurotransmitter diseases serve as a proof of concept revealing the magnetic sensitization. The detection limit of the 5-HT was decreased to 3.21 nM in the linear range of 5-500 nM, which was lower than that of the biosensor without magnetic field. The clinical 5-HT samples were also been accurately detected, indicating a positive application potential of the as-prepared magnetic-mediated biosensor. All these results reveal the high sensitivity and practicality of the as-prepared magnetic-mediated biosensor and provide a new method for neurotransmitter diseases diagnosis.

Keywords: magnetic mediation, sensitization, 5-HT detection, biosensor