CRISPR-based diagnostic in aquaculture: Application, Potential/Opportunities, and Limitations

28 Pages Posted: 7 May 2024

See all articles by Kailash Bohara

Kailash Bohara

University of Arkansas at Pine Bluff - Aquaculture/Fisheries Center

Ali Parsaeimehr

Delaware State University; Instituto Tecnológico de Monterrey

Sujan Bhattarai

University of Arkansas at Pine Bluff - Aquaculture/Fisheries Center

Date Written: May 2, 2024

Abstract

Aquaculture is an expanding industry encompassing both inland and sea-based operations, driven by the increasing demand for aquaculture products. However, the occurrence of various diseases in the aquaculture industries leads to substantial economic losses amounting to billions of dollars annually. Rapid diagnosis of diseases is crucial for the early surveillance and treatment of aquaculture diseases. Although nucleic acid-based diagnostic methods are currently utilized in aquaculture, they suffer from prolonged turnaround times and a lack of efficient diagnostic techniques, which hinders the management and treatment of aquatic diseases. In contrast, CRISPR-based diagnostic methods have emerged as promising alternatives due to their portability, sensitivity, rapidity, and cost-effectiveness, as demonstrated in human and animal diagnostics. Consequently, researchers have dedicated efforts in recent years to develop various assays for diagnosing aquatic diseases, capitalizing on diverse CRISPR-Cas systems, each with their own strengths and limitations. This review aims to consolidate and summarize the characteristics, applicability, and limitations of different CRISPR-based diagnostic methods developed specifically for aquaculture.

Keywords: Fish disease, Aquaculture, Multiplex CRISPR, Amplification, Cas12a

Suggested Citation

Bohara, Kailash and Parsaeimehr, Ali and Bhattarai, Sujan, CRISPR-based diagnostic in aquaculture: Application, Potential/Opportunities, and Limitations (May 2, 2024). Available at SSRN: https://ssrn.com/abstract=4815342 or http://dx.doi.org/10.2139/ssrn.4815342

Kailash Bohara (Contact Author)

University of Arkansas at Pine Bluff - Aquaculture/Fisheries Center ( email )

Pine Bluff, AR
United States

Ali Parsaeimehr

Delaware State University ( email )

Dover, DE 19901
United States

Instituto Tecnológico de Monterrey ( email )

School of Engineering and Sciences, Tecnologico de
Monterrey, Nuevo León
Mexico

HOME PAGE: http://https://mx.linkedin.com/in/ali-parsaeimehr-822b173a

Sujan Bhattarai

University of Arkansas at Pine Bluff - Aquaculture/Fisheries Center ( email )

Pine Bluff, AR
United States

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