Impact of Trace Elements Added to Anaerobic Digestion Reactors on Their Activity and Microbial Community Structure

34 Pages Posted: 7 May 2024

See all articles by Rahul Honavar Melo Pires

Rahul Honavar Melo Pires

affiliation not provided to SSRN

Maria Paola Tomasino

affiliation not provided to SSRN

Stéphane Simon

affiliation not provided to SSRN

Rémy Buzier

affiliation not provided to SSRN

Gilles GUIBAUD

affiliation not provided to SSRN

C. Marisa R. Almeida

affiliation not provided to SSRN

Ana Paula Mucha

affiliation not provided to SSRN

Abstract

The effects of trace elements (TEs) addition (Cu, Ni, Co, Cd) on anaerobic digestion (AD) were studied in lab-scale experiments. Reactor performance was assessed by monitoring biogas production. The labile fraction of TEs (the most bioaccessible species) was determined by diffusive gradients in thin-films technique. Prokaryotic community composition was characterized through high throughput sequencing (HTS) targeting archaea and bacteria and qPCR was used to evaluate changes in methanogens and metal resistance genes. Even if only a minor fraction of spiked concentration was labile, all TEs induced inhibitory effects at the highest spiking concentration (100 μM). Only Cd and Ni exhibit inhibition at lower concentrations. HTS and qPCR revealed changes, particularly in archaea, with reduced relative abundance at higher TE concentrations. Shifts in prokaryotic communities suggest alterations in AD metabolic pathways. This study enhances our understanding of TEs' influence on prokaryotic communities in AD, providing insights for optimal dosing.

Keywords: anaerobic digestion, Trace elements, High Throughput Sequencing, Microbial community, Metal resistance genes

Suggested Citation

Pires, Rahul Honavar Melo and Tomasino, Maria Paola and Simon, Stéphane and Buzier, Rémy and GUIBAUD, Gilles and Almeida, C. Marisa R. and Mucha, Ana Paula, Impact of Trace Elements Added to Anaerobic Digestion Reactors on Their Activity and Microbial Community Structure. Available at SSRN: https://ssrn.com/abstract=4819852 or http://dx.doi.org/10.2139/ssrn.4819852

Rahul Honavar Melo Pires (Contact Author)

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Maria Paola Tomasino

affiliation not provided to SSRN ( email )

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Stéphane Simon

affiliation not provided to SSRN ( email )

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Rémy Buzier

affiliation not provided to SSRN ( email )

No Address Available

Gilles GUIBAUD

affiliation not provided to SSRN ( email )

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C. Marisa R. Almeida

affiliation not provided to SSRN ( email )

No Address Available

Ana Paula Mucha

affiliation not provided to SSRN ( email )

No Address Available

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