Photothermal Enhanced Antibacterial Chitosan-Based Polydopamine Composite Hydrogel for Hemostasis and Burn Wound Repairing

Abstract

Bleeding and bacterial infection are common problems associated with wound treatment, while effective blood clotting and vessel regeneration promotion are the primary considerations to design the wound dressing materials. This research presents a chitosan-based hydrogel with grafted quaternary ammonium and polyphosphate (QCSP hydrogel) as the antibacterial hemostatic dressing to achieve burn wound treatment. The tissue adhesion of the hydrogel sealed the blood flow and the polyphosphate grafted to the chitosan promoted the activation of coagulation factor V to enhance the hemostasis. At the same time, the grafted quaternary ammonium enhanced the antibacterial ability of the biodegradable hydrogel wound dressing. In addition, the polydopamine as a photothermal agent was composited into the hydrogel to enhance the antibacterial and reactive oxygen scavenging performance. The in vivo hemostasis experiment proved the polyphosphate enhanced the coagulation property. Moreover, this photothermal property of the composite hydrogel enhanced the burn wound repairing rate combined with the NIR stimulus. As a result, this hydrogel could have potential application in clinic as dressing material for hemostasis and infection prone would repairing.

Note:
Funding declaration: This work was supported by the National Natural Science Foundation of China (82072327), State Key Laboratory of New Pharmaceutical Preparations and Excipients, State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University (FAMP202006k) and the Natural Science Foundation of Hebei Province (No. C2023201073, H2021423064).

Conflict of Interests: The authors declare no competing financial interest.

Ethical Approval: All animal experiments were carried out according to the ARRIVE guidelines in accordance with National Research Council's Guide for the Care and Use of Laboratory Animals (8th edition), and approved by Hebei University AWEC (SYXK(Ji)2022-009).

Keywords: Chitosan, Hydrogel, Photothermal property, Hemostasis, Wound healing