Metabolic Pathways in Off-Flavor Mitigation within Recirculating Aquaculture Systems

Abstract

This study explores the dissolution and metabolism of off-flavors in Sebastes schlegelii, a significant species in recirculating aquaculture systems (RAS). We conducted an experimental investigation across varied temperatures and flow rates to understand the impact of RAS on off-flavor metabolism. Using UPLC-MS/MS metabolomics, we identified key metabolic changes, including altered levels of specific metabolites like L-phenylalanine, L-isoleucine, glucuronic acid, L-aspartic acid, and L-lysine. These changes were linked to significant metabolic pathways, such as amino acid biosynthesis and hemoglobin metabolism. Principal component analysis indicated significant variations in muscle metabolic profiles under different RAS conditions. Our findings highlighted key metabolic pathways influenced by RAS conditions, including amino acid biosynthesis, hemoglobin etabolism, the tricarboxylic acid cycle, and the lysine degradation pathway. This research provides valuable insights for optimizing fish quality in RAS by elucidating the metabolic responses of Sebastes schlegelii to different RAS conditions.

Keywords: Geosmin(GSM), 2-methylisobornyl (2-MIB), Recirculating aquaculture systems, Metabolomics, Sebastes schlegelii