Proteomics and Antioxidant Activity Analysis of Polygonatum Cyrtonema Hua, P. Kingianum Coll. Et Hemsl. And P. Sibiricum Red

Polygonati Rhizoma is an essential and edible crop in China. In this study, we focused on the property analysis of protein contents of Polygonatum kingianum Coll. et Hemsl, P. cyrtonema Hua, and P. sibiricum Red. Glu, Arg, His, and Asp were the main AAs in the P. Rhizoma samples. The P. cyrtonema samples exhibited the highest quantity of protein types, and P. kingianum was the lowest. The functional enrichment results presented enrichment pathways related to carbohydrate, energy, and AA metabolism. We found that almost all antioxidant peptide sequences, except EL and AGDDAPR, were broke up after the predicted hydrolysis treatment. The molecular docking results demonstrated that both EL and AGDDAPR could hinder the potential binding of Keap1 to Nrf2 through stably binding to Keap1 with -5.3 and -6.0 kcal/mol. Our research could provide an essential reference for fully utilizing the P. Rhizoma protein component.

Keywords: Polygonati Rhizoma, protein, antioxidant activity, Hydrolyzed amino acids, proteomics, Molecular docking

Suggested Citation: Suggested Citation

liu, chenxing and Cao, Yan and Zhao, Yuqi and Lu, Shengmin and Xia, Qile, Proteomics and Antioxidant Activity Analysis of Polygonatum Cyrtonema Hua, P. Kingianum Coll. Et Hemsl. And P. Sibiricum Red. Available at SSRN: https://ssrn.com/abstract=4861603 or http://dx.doi.org/10.2139/ssrn.4861603