Effects of Water Temperature Increase on Transcriptomic, Biochemical, and Histopathological Responses of the Bivalve Species Mytilus Galloprovincialis to the Antineoplastic Drug  5-Fluorouracil

43 Pages Posted: 21 Jun 2024

See all articles by Vanessa Queirós

Vanessa Queirós

affiliation not provided to SSRN

Carla Leite

Universidade de Aveiro

Ulisses M. Azeiteiro

affiliation not provided to SSRN

Marta Casado

affiliation not provided to SSRN

Amadeu M.V.M. Soares

Universidade de Aveiro - Department of Biology

Juan Luis Santos

University of Seville

Esteban Alonso

University of Seville

Rosa Freitas

Universidade de Aveiro - Department of Biology

Carlos Barata

Institute of Environmental Assessment and Water Research (IDAEA-CSIC)

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Abstract

The present study aimed to assess the potential ecotoxicological effects of 5-fluorouracil (5-FU), an antineoplastic already found in aquatic ecosystems, towards the bivalve Mytilus galloprovincialis, considering actual and warming temperature scenarios. To this end, organisms were exposed for 28 days to increasing 5-FU concentrations (10, 100, and 500 ng/L) at control (17 ± 1.0 °C) and warming (21 ± 1.0 °C) temperatures. Transcriptomic of selected target genes, biochemical responses, and histopathological alterations were used to assess possible detrimental effects. Results showed an overall down-regulation of mRNA transcripts related to xenobiotic metabolization pathways and cell apoptosis in organisms exposed to 5-FU, especially at 21 ºC. While at 17 ºC, the antioxidant defenses were not enough to avoid cell damage caused by 5-FU, at 21 ºC the metabolic slowdown avoided further cell damage. However, histopathological alterations in the digestive gland at 21 ºC and high concentrations of 5-FU were observed. Overall, the present results indicate that the warming temperature may enhance the detrimental effects of 5-FU on this species, with metabolism being particularly affected.

Keywords: Anticancer drugs, Bivalves, climate change, Gene expression, Oxidative stress

Suggested Citation

Queirós, Vanessa and Leite, Carla and Azeiteiro, Ulisses M. and Casado, Marta and Soares, Amadeu M.V.M. and Santos, Juan Luis and Alonso, Esteban and Freitas, Rosa and Barata, Carlos, Effects of Water Temperature Increase on Transcriptomic, Biochemical, and Histopathological Responses of the Bivalve Species Mytilus Galloprovincialis to the Antineoplastic Drug  5-Fluorouracil. Available at SSRN: https://ssrn.com/abstract=4872617 or http://dx.doi.org/10.2139/ssrn.4872617

Vanessa Queirós

affiliation not provided to SSRN ( email )

No Address Available

Carla Leite

Universidade de Aveiro ( email )

Ulisses M. Azeiteiro

affiliation not provided to SSRN ( email )

No Address Available

Marta Casado

affiliation not provided to SSRN ( email )

No Address Available

Amadeu M.V.M. Soares

Universidade de Aveiro - Department of Biology ( email )

Juan Luis Santos

University of Seville ( email )

Avda. del Cid s/n
Sevilla, 41004
Spain

Esteban Alonso

University of Seville ( email )

Avda. del Cid s/n
Sevilla, 41004
Spain

Rosa Freitas

Universidade de Aveiro - Department of Biology ( email )

Carlos Barata (Contact Author)

Institute of Environmental Assessment and Water Research (IDAEA-CSIC) ( email )

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