Developmental Validation of the Idseek® Omnistr™ Global Autosomal Str Profiling Kit

18 Pages Posted: 18 Jul 2024

See all articles by Pieter van Oers

Pieter van Oers

affiliation not provided to SSRN

Erik A.C. de Jong

affiliation not provided to SSRN

Melanie Arts

affiliation not provided to SSRN

Kristiaan J. van der Gaag

Netherlands Forensic Institute

Joop Theelen

affiliation not provided to SSRN

Abstract

AbstractForensic science takes advantage of population variability in autosomal Short Tandem Repeat (STR) lengths to establish human identification. The most common method for DNA profiling by STR is based on PCR, where the highly polymorphic STR regions are amplified and analysed using Capillary Electrophoresis (CE) or Massively Parallel Sequencing (MPS). MPS determines not only the repeat length, but also the repeat structure and variations in the flanking regions, making this method superior in discriminatory power compared to CE. Reverse Complement PCR (RC-PCR) is a novel, more sophisticated PCR based MPS library preparation method combining indexing and PCR amplification in a single closed-tube reaction. In this document we describe the complete developmental validation of the IDseek® OmniSTR™ kit, a RC-PCR based MPS library preparation kit. The developed IDseek® OmniSTR™ kit contains 28 autosomal STR targets, one Y-chromosomal STR and the Amelogenin gene covering all relevant STR core loci from the USA, EU, UK and Interpol.

Keywords: RC-PCR, MPS, STR, Forensic DNA, human identification, OmniSTR™, IDseek®

Suggested Citation

van Oers, Pieter and de Jong, Erik A.C. and Arts, Melanie and van der Gaag, Kristiaan J. and Theelen, Joop, Developmental Validation of the Idseek® Omnistr™ Global Autosomal Str Profiling Kit. Available at SSRN: https://ssrn.com/abstract=4898314 or http://dx.doi.org/10.2139/ssrn.4898314

Pieter Van Oers (Contact Author)

affiliation not provided to SSRN ( email )

No Address Available

Erik A.C. De Jong

affiliation not provided to SSRN ( email )

No Address Available

Melanie Arts

affiliation not provided to SSRN ( email )

No Address Available

Kristiaan J. Van der Gaag

Netherlands Forensic Institute ( email )

Netherlands

Joop Theelen

affiliation not provided to SSRN ( email )

No Address Available

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