Electrochemical-Induced C-O Bond Formation Targeting Bioconjugation and Its Application in Osteogenesis

Abstract

It is of great significance in the fields of chemical biology and pharmacology to directly introduce small molecules containing medicinal value into biomolecules under clean, mild, and easy-to-operate conditions to achieve the effect of enhancing the expression of original biomolecules. Here, we report a method for targeted bioconjugation of electrically induced C-O bond formation to perform the bioconjugation of aniline derivatives with peptides containing tyrosine residues in a rapid and efficient manner. In this electrochemical system, various biomolecules and peptides are successfully labeled with good targeting and chemical selectivity. In addition, this technology experimentally verified through in vitro and in vivo experiments that the bioconjugation of DMpT-OGP not only preserves its biocompatibility but also significantly boosts its ability to promote cell proliferation and osteogenic differentiation.

Keywords: Tyrosine, Electrochemical-induced, C-O Bond Formation, Peptides Modifica-tion, Drug Molecules, Bone Growth & Repair