Type I Interferon (Ifnd) Enhanced the Innate Immune Response and Induced Lipid Droplets Formation in Obscure Puffer Takifugu Obscurus

Abstract

As one member of type I IFN, IFNd showed huge distinctive responses and activity during the viral or bacterial infection in various fish species. Our previous study identified IFNd from obscure puffer Takifugu obscurus (ToIFNd), and the function and regulation of ToIFNd was further in the present study. The transcriptional levels of ToIFNd were significantly induced post bacterial and poly (I:C) stimulation in the head kidney. In addition, recombinant ToIFNd (rToIFNd) treatment enhanced the expressions of numerous interferon stimulated genes (ISGs, such as Mx1, PKR1, and PKR2) and several immune effectors (such as Il1, faslg, and tnf14) both in the in vivo and in vitro experiments. The expression patterns of interferon regulatory factors (IRFs) post rToIFNd stimulation suggesting that IRF3, 7, and 8 might play critical role during the ToIFNd mediated signal transduction. Remarkably, we firstly found that rToIFNd stimulation promoted lipid droplets (LDs) formation in liver, which was further confirmed by the increased expressions of LDs marker PLIN3, as well as the transcripts of genes involved in the transport and synthesis of neutral lipids. However, the possible interaction between the ToIFNd and LDs formation require further study. These results corroborate the antibacterial and antiviral function of ToIFNd in obscure puffer, which will contribute to explore the functional characteristics of IFN system.

Keywords: IFNd, Obscure puffer (Takifugu obscurus), Interferon stimulated genes (ISGs), Interferon regulatory factors (IRFs), Lipid droplets (LDs)