A Facile Dual-Signal Ratiometric Sensor of Creatinine Based on Silver Nanoparticles/Mxenes with Intrinsic Self-Calibration Property for Thionine Acetate

Abstract

In this work, a ratiometric double signals electrochemical sensing platform was constructed for creatinine(Cre) analyse employing silver nanoparticles(AgNPs)@ MXenes/thionine acetate(THAT) composites. The synthesis of MXenes/THAT composites was utilized to modify on the surface of a screen-printed electrode(SPE) via dripping coating, followed by the electro-deposition of AgNPs . While the anodic current of the AgNPs decreased in the presence of Cre due to the competive reaction for forming metal complexes, the current of THAT remained invariant, which attained a ratiometric response. Under optimized conditions, the response current ratio (IAg/ITHAT) decreases while the concentration of Cre increased linearly between 0~1.2mM, with 0.01 μM as a adaptive detection limit. It provided a new sensing platforming for employing double-signals nanostructures with remarkable stability and sensitivity to design ratiometic sensors for analysis of bio-molecule in human urea samples with real-time.

Keywords: ratiometric sensor， electrochemical sensing，creatinine analyse，self-calibration