Capturing Co2 and Flue Gas Carbon by Methanosarcina Mazei Go1 and Methanothermobacter Thermautotrophicus Δh

Abstract

In this study, the specific methanogenic activities of mesophilic Methanosarcina mazei Go1 and thermophilic Methanothermobacter thermautotrophicus DH were compared to capture CO2 and flue gas M. thermautotrophicus showed higher methanogenic activity in the reactors supplemented with flue gas (13.46±0.538 µmol/L) and CO2 (12.76±0.510 µmol/L) than M. mazei. Optimal hydrogenotrophic methanogenesis of these methanogens observed at pH 8 and 150 kPa. Methanogenic activity increased with increasing the inoculum size of M. mazei at 37oC and decreasing the inoculum size of M. thermautotrophicus at 65oC. M. mazei and M. thermautotrophicus preferred tryptone and peptone for bioconversion of  flue gas and CO2 to methane, respectively. (11.70±0.468 µmol/L) by both organisms. K2HPO4 and Na2HPO4 M. thermautotrophicus exhibited the highest methanogenic activity (18.64±0.745 µmol/L) when flue gas was carbon source. It was better than that produced by M. mazei (9.41±0.376 µmol/L) under the same conditions. methane production from CO2 and flue gas using these organisms.

Keywords: Methanogens, Methanosarcina, Methanothermobacter, Biogas, Carbon capturing, Methanogenic bioprocessing