The Structural Discrepancy between the Ability of Two Neutral Heteropolysaccharides to Cure Acute Pharyngitis in Hosta Plantaginea (Lam.) Ascher Flowers

Abstract

The polysaccharides (HPFP-1 and HPFP-2) were obtained from Hosta plantaginea (Lam.) Aschers flowers by separation. After structural analysis, HPFP-1 was determined to be composed of glucose and fructose at a molar ratio of 17.08:82.92. The structure was as follows: →1)-β-D-Fruf-(2→ and →1,6)-β-D-Fruf-(2→, →6)-α-D-Glcp were interconnected to form the main chain, and the branches consisted of β-D-Fruf-(2→ attached to the O-6 position of the glycosyl residue →1,6)-β-D-Fruf-(2→ of the main chain. HPFP-2 was composed of arabinose, rhamnose, galactose, glucose, xylose, mannose, and glucuronic acid at a molar ratio of 24.90:6.87:59.56:3.03:2.29:1.38:1.95. The glycosyl residue →3,6)-β-D-Galp-(1→, →5)-α-L-Araf-(1→, →3)-β-D-Galp-6-OMe(1→, →6)-β-D-Galp-(1→, →4)-α-D-Galp-(1→ were interconnected to form the main chain of HPFP-2. The branches were comprised of α-L-Araf-(1→ and β-D-Galp-(1→ attached to the O-3 position of the main chain different glycosyl residue →3,6)-β-D-Galp-(1→. In acute pharyngitis rats, HPFP effectively increased the level of NO, decreased the levels of TNF-α, IL-1β, and IL-6, reduced macrophage and neutrophil infiltration. In the LPS-induced RAW 264.7 cell model, HPFP-1 and HPFP-2 decreased the levels of NO, TNF-α, IL-6, p-p65, and p-IκB, which demonstrated the anti-inflammatory mechanism of these two polysaccharides by inhibition of the NF-κB signaling pathway. Therefore, they have the potential to be highly effective drugs combating acute pharyngitis.

Keywords: Hosta plantaginea (Lam.) Aschers, Polysaccharide, structure, Acute pharyngitis