Low Isopentenyl Transferase Activity Inhibited Apical Silk Elongation Under Heat and Drought Stress

30 Pages Posted: 18 Oct 2024

See all articles by Ruixin Shao

Ruixin Shao

Henan Agricultural University

Hongwei Li

Henan Agricultural University

Yulou Tang

Henan Agricultural University

Fanzheng Meng

Henan Agricultural University

Wenqi Zhou

affiliation not provided to SSRN

Wei Liang

Henan Agricultural University

Junwei Yang

China Agricultural University

Yongchao Wang

Henan Agricultural University

Hao Wang

Henan Agricultural University

Jiameng Guo

Henan Agricultural University

Qinghua Yang

Henan Agricultural University

Abstract

The impact of heat and drought stress on the development of maize reproductive organs is unclear. In this study, heat and drought stress during the ear development stage decreased pollen and silk viability, leading to inhibited apical silk elongation and floret abortion, which further increased the unfertilized silk ratio and reduced the number of kernels. Pollen and silk metabolome analysis revealed that soluble sugar, including sucrose, D-fructose, D-galactose, and D-glucose, changed dramatically in pollen under stress, and cytokinin was the sole phytohormone significantly positively correlated with silk viability under stress. In addition, transcriptome profiling in the crosstalk pathway between zeatin and sugar biosynthesis showed that the lower expression of Zm00001d047253-encoded sucrose synthase enzyme suppressed the transformation of sucrose into D-fructose, possibly resulting in decreased pollen and silk viability under stress. Moreover, low transcript levels of Zm00001d038921 and Zm00001d034584, which encode isopentenyl transferase, in silk restricted cytokinin biosynthesis under stress. Low isopentenyl transferase activity decreased cytokinin content resulting in dwarf silks under stress and exogenous application of cytokinin significantly restored the apical silks under drought stress. These findings provide new insights for breeding maize that is better adapted to climate change to maintain global food security.

Keywords: Abiotic stress, Multi-omics, Cytokinin, Food security

Suggested Citation

Shao, Ruixin and Li, Hongwei and Tang, Yulou and Meng, Fanzheng and Zhou, Wenqi and Liang, Wei and Yang, Junwei and Wang, Yongchao and Wang, Hao and Guo, Jiameng and Yang, Qinghua, Low Isopentenyl Transferase Activity Inhibited Apical Silk Elongation Under Heat and Drought Stress. Available at SSRN: https://ssrn.com/abstract=4992129 or http://dx.doi.org/10.2139/ssrn.4992129

Ruixin Shao (Contact Author)

Henan Agricultural University ( email )

Zhengzhou
China

Hongwei Li

Henan Agricultural University ( email )

Zhengzhou
China

Yulou Tang

Henan Agricultural University ( email )

Zhengzhou
China

Fanzheng Meng

Henan Agricultural University ( email )

Zhengzhou
China

Wenqi Zhou

affiliation not provided to SSRN ( email )

No Address Available

Wei Liang

Henan Agricultural University ( email )

Zhengzhou
China

Junwei Yang

China Agricultural University ( email )

Beijing
China

Yongchao Wang

Henan Agricultural University ( email )

Zhengzhou
China

Hao Wang

Henan Agricultural University ( email )

Zhengzhou
China

Jiameng Guo

Henan Agricultural University ( email )

Zhengzhou
China

Qinghua Yang

Henan Agricultural University ( email )

Zhengzhou
China

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