High-Throughput Screening for Respiratory Pathogens within Pigs in Denmark; Analysis of Circulating Porcine Respiratory Coronaviruses and Their Association with Other Pathogens

37 Pages Posted: 22 Oct 2024

See all articles by Amalie Ehlers Bedsted

Amalie Ehlers Bedsted

affiliation not provided to SSRN

Nicole B. Goecke

affiliation not provided to SSRN

Charlotte K. Hjulsager

Statens Serums Institut

Pia Ryt-Hansen

University of Copenhagen

Kasama Chusang Larsen

affiliation not provided to SSRN

Thomas Bruun Rasmussen

Statens Serums Institut

Anette Bøtner

affiliation not provided to SSRN

Lars Erik Larsen

University of Copenhagen

Graham John Belsham

affiliation not provided to SSRN

Abstract

Porcine respiratory coronavirus (PRCV) typically causes subclinical or mild respiratory infections in pigs, but may lead to more severe disease with other factors. PRCV infection in Denmark was initially detected in 1984, but data are lacking about its current prevalence and diversity. Antibodies against PRCV were detected in about 75% of recent pig sera from Denmark. In addition, pig nasal swab samples were screened for PRCV and 12 other pathogens using a high-throughput RT-qPCR system. All targeted pathogens were detected but at different prevalences. Significant associations were found between the presence of PRCV and certain other pathogens. From PRCV positive samples, partial spike gene sequences and complete nucleocapsid coding sequences were determined. In phylogenetic analyses, these PRCVs clustered with earlier European PRCVs and were distinct from transmissible gastroenteritis virus. We conclude that PRCV is widespread within the pig population in Denmark. Further studies on the significance of PRCV are warranted.

Keywords: Porcine respiratory disease complex (PRDC), porcine respiratory coronavirus (PRCV), swine influenza A virus (swIAV), respiratory bacterial pathogens, Coronaviruses

Suggested Citation

Bedsted, Amalie Ehlers and Goecke, Nicole B. and Hjulsager, Charlotte K. and Ryt-Hansen, Pia and Larsen, Kasama Chusang and Rasmussen, Thomas Bruun and Bøtner, Anette and Larsen, Lars Erik and Belsham, Graham John, High-Throughput Screening for Respiratory Pathogens within Pigs in Denmark; Analysis of Circulating Porcine Respiratory Coronaviruses and Their Association with Other Pathogens. Available at SSRN: https://ssrn.com/abstract=4994953 or http://dx.doi.org/10.2139/ssrn.4994953

Amalie Ehlers Bedsted

affiliation not provided to SSRN ( email )

No Address Available

Nicole B. Goecke

affiliation not provided to SSRN ( email )

No Address Available

Charlotte K. Hjulsager

Statens Serums Institut ( email )

Pia Ryt-Hansen

University of Copenhagen ( email )

Nørregade 10
Copenhagen, DK-1165
Denmark

Kasama Chusang Larsen

affiliation not provided to SSRN ( email )

No Address Available

Thomas Bruun Rasmussen

Statens Serums Institut ( email )

Anette Bøtner

affiliation not provided to SSRN ( email )

No Address Available

Lars Erik Larsen

University of Copenhagen ( email )

Nørregade 10
Copenhagen, DK-1165
Denmark

Graham John Belsham (Contact Author)

affiliation not provided to SSRN ( email )

No Address Available

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