Ratiometric Sensors Based on Ag+-Mediated Luminescence of Tb-Dna Complexes for Visual Detection of 4-Aminophenol

Abstract

Development of accurate, convenient and portable methods for monitoring 4-aminophenol (4-AP) is extremely important because of its strong toxicity. Here, a ratiometric fluorescence sensor based on Ag+-enhanced luminescence of Tb-DNA complexes has been presented for the detection of 4-AP. The luminescence of Tb-DNA complexes is enhanced about 30 times by Ag+, which can trigger energy transfer from DNA to Tb3+ more efficiently. In the presence of 4-AP, Ag+ can be reduced into Ag0 owing to its strong reducibility, inducing the decrease of Tb-DNA complexes at 545 nm remarkably. Besides, 4-AP exhibits intrinsic fluorescence at 375 nm under the same conditions. Therefore, ratiometric detection of 4-AP can be achieved using F375/F545 as readout with a detection limit of 0.4 M. Real water samples have been tested to evaluate this method in practical application and satisfactory results have been obtained. Furthermore, the addition of 4-AP into Tb-DNA-Ag+ system induces a visible color variation from green to bluish violet, enabling visual detection of 4-AP under the assistance of a smartphone.

Keywords: Ratiometric fluorescence, Terbium ion, DNA, Silver ion, 4-Aminophenol, Visual detection