Melatonin Protects Aged Oocytes from Depalmitoylation-Mediated Quality Reduction by Promoting Ppt1 Degradation and Antioxidation

Abstract

Oocyte aging is closely related to a decline in female fertility, accompanied by increased reactive oxygen species levels and changes in protein posttranslational modifications. However, the role of protein palmitoylation in oocyte aging has not been investigated. In the present study, a new association between redox and palmitoylation in aging oocytes was found. We found that the protein level of palmitoyl-protein thioesterase 1 (PPT1), a depalmitoylation enzyme, was increased in maternally aged mice oocytes and follicular fluid of aged-DOR patients. Elevated PPT1 leads to decreased S-palmitoylation of oocyte, which impairs oocyte maturation and spindle formation. Tubulin was identified as a critical palmitoylated protein regulated by PPT1, whose palmitoylation was also decreased by advanced age, accompanied by abnormalities in membrane localization and microtubule polymerization. Melatonin was found to down-regulate excessive PPT1 and rescue PPT1-induced damage in mouse oocytes, not only by regulating oxidative stress, but also by binding with PPT1 to regulate its lysosomal degradation. In summary, our data demonstrate that PPT1 participates in oocyte aging by regulating tubulin palmitoylation, providing evidence that oxidative stress regulates protein palmitoylation and revealing a novel mechanism of oocyte aging.

Note:
Funding declaration: This work was supported by the National Natural Science Foundation (82271687, 82301869, U22A20277, 82274651, 81973965, 32000583 and 81971373), the Jiangsu Key Research and Development Program (Grant No. BE2022712), the Key Project of Medical Scientific Research of Jiangsu Commission of Health (ZD2022004), and Jiangsu Provincial Medical Key Discipline Cultivation Unit (JSDW202215).

Conflict of Interests: The authors declare that there are no competing interests.

Ethical Approval: This human study was approved by the Ethics Committee of Jinling Hospital (2022DZGDR-035) and was performed in accordance with national and international guidelines.

Keywords: oocyte aging, Melatonin, antioxidation, palmitoyl-protein thioesterase 1, palmitoylation, tubulin