Identification and Functional Analysis of Proteins in Anabarilius Grahami At Different Growth Stages Based on 4d-Dia Quantitative Proteomics Technology

Abstract

In this study, 4D-DIA quantitative proteomics technology was used to investigate the proteome of Anabarilius grahami at different growth stages, i.e., three-year-old (K3), five-year-old (K5), and seven-year-old (K7), which compared to Carassius auratus. A total of 1,821 proteins were identified, in which six differentially abundant proteins (DAPs) could be considered as markers to discriminate C. auratus and A. grahami. Additionally, seven DAPs were identified, which could help to distinguish A. grahami throughout the different growth stages. In addition, AST and ALT were identified as being associated with the synthesis of Glu and Asp, while HACD and CZIB were involved in the synthesis of PUFA, including DHA and EPA. These findings provide deeper insights into the nutritional and functional properties of the proteins in A. grahami, and may serve as the basis for the next steps on the evaluation of the nutritional and flavor quality of A. grahami.

Keywords: Anabarilius grahami, 4D-DIA quantitative proteomics, Protein function, synthesis of umami amino acids, PUFA synthesis