Biomimetic Enzyme Electrochemical Sensor Based on Cu-Doped Zif-8 and Calcined Uio-66 for Rapid and Sensitive Detection of Zearalenone in Vegetable Oil Deodorizer Distillate

Abstract

In order to ensure the safety of the raw material vegetable oil deodorizer distillate (VODD) using in vitamin E and phytosterols production process, it is necessary to rapidly and sensitively detect the content of Zearalenone mycotoxins. In this study, a novel biomimetic enzyme electrochemical sensor of Cu-ZIF-8@CN-UIO-66 was developed. The surface morphology, microstructure, elemental composition, chemical state, crystal structure and functional groups of biomimetic nano-enzyme were studied by SEM, EDS, XPS, XRD and FT-IR. The linear range of the biosensor for ZEN detection was 10 ng mL-1-25 μg mL-1, and the detection limit was as low as 0.6 ng mL-1. Meanwhile, in the actual sample detection of VODD, the recovery rate was 95.9% -102.2%. The above experiment result indicated that the prepared composite nano-biomimetic enzyme was an ideal choice for the detection of ZEN in VODD and showed a broad application prospect in mycotoxin analysis.

Keywords: Electrochemical sensor, Nanobiomimetic enzyme, Zearalenone, Fast sensitive detection