Amino-Functionalized Ce-Mof Synthesized by Micro-Plasma: As a Dual-Mode Colorimetric/Fluorescence Sensor for On-Site Analysis of Doxycycline

Abstract

The improper and excessive use of doxycycline (DOX) poses a significant risk to public health through food residues and soil/water contamination, however on-site and accurately distinguish DOX from complex substrates (especially other tetracycline antibiotics which with similar molecular structures) remains a significant challenge for field analysis. In this work, an amino-functionalized cerium-based metal-organic framework (NH2-Ce-MOF) with high peroxidase activity was one-step synthesized by dielectric barrier discharge microplasma. The DOX adsorbs more readily on the (001) crystal faces of NH2-Ce-MOF compared with other tetracyclines, thus facilitating its distinct recognition. Based on the inward filtration effect (IFE) and selective adsorption between NH2-Ce-MOF and TMB, a high effective dual-mode colorimetric/fluorescent DOX sensor was developed and applied for on-site analyzing really pharmaceutical and livestock sewage samples by smartphone assisted visualization, with a concentration range of 0.1-150 μM and a low limit of detection (LOD) of 79 nM with recoveries ranging from 96.56%-107.22%.

Keywords: NH2-Ce-MOF, microplasma, Dual-mode sensing, On-site analysis, livestock sewage