Engineering Robust Saccharomyces Cerevisiae Cell Factory for Improving Fatty Alcohol Biosynthesis

Abstract

Engineering yeast cell factories is a feasible approach to produce value chemicals from renewable feedstocks. However, during the production process, reprogramming of the internal metabolic pathways of yeast cells and environmental stress always compromises its production performance. Here, we engineered the robustness Saccharomyces cerevisiae to enhance the production of fatty alcohols by downregulating the expression of target of rapamycin gene TOR1 and deleting histone deacetylase gene HDA1 in S. cerevisiae. In fact, the efficiency of the yeast cell factory is determined by its metabolic capacity, physiological state and environmental conditions, and increasing the weight of one of these is bound to promote cell performance.

Keywords: Yeast, Robustness, Fatty alcohol, Cell performance