Searching for Bacteria within Acute Cholecystitis Using Next-Generation Sequencers
19 Pages Posted: 24 Mar 2025 Publication Status: Under Review
Abstract
Introduction: A biliary microbiome comprising flora within normal gallbladders was recently uncovered through analyses targeting the bacterial 16S ribosomal RNA (16S rRNA) gene, despite the gallbladder previously being regarded as a sterile environment. In the present study, we subjected bile samples from patients with acute cholecystitis to gene analysis targeting bacterial flora.
Note:
Funding declaration: This work was supported by Asahi Group Holdings, Ltd. The funder had no role in the study design, in the collection, analysis, or interpretation of data, in the writing of the report, or in
the decision to submit the article for publication.
Conflict of Interests: This study received funding from Asahi Group Holdings, Ltd. The authors declare no remaining conflicts of interest.
Ethical Approval: Informed consent was obtained from all individual participants included in the study. The study was approved by the ethics committee of Juntendo University, Urayasu Hospital (Approval No. 3-009; 13 March 2021).
Keywords: Acute cholecystitis, Bacteria flora, Next-generation sequencer, 16S ribosomal RNA, 16S rRNA, Biliary microbiome
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