Synthesis of Cof (Tapa-Dhta)-Sodium Alginate-Ca2+-Polyacrylate Composite Microspheres as Adsorbent for Dispersive Solid Phase Extraction of Fluoroquinolone Antibiotics in Milk Prior to the Quantification by Hplc-Ms/Ms

This work developed hydroxyl-functionalized covalent organic framework (COF) via ambient-temperature synthesis and integrated it with sodium alginate-Ca2+-polyacrylate hydrogel to form composite microspheres (CACMPs) for dispersive solid-phase extraction (dSPE) of five fluoroquinolone antibiotics in milk. Combining COF with hydrogel can effectively improve the hydrophilicity of adsorbent. The CACMPs do not require centrifugation and can be completely separated within one minute. After five cycles, they still maintain high extraction efficiency (>90%). Coupled with HPLC-MS/MS, the method achieved a wide linear range (0.01–100 ng/mL), low detection limits (0.05–0.39 μg/kg), and high precision (RSD ≤8.8%). Validation via spiked milk samples yielded recoveries of 81.6–112.1%, confirming reliability for monitoring FQ residues in dairy. Design of CACMPs combines COF adsorption capacity with hydrogel practicality, offering a sustainable, efficient solution for antibiotic analysis in complex matrices.

Keywords: Covalent organic framework, Sodium alginate, Hydrogel, Fluoroquinolones, HPLC-MS/MS

Suggested Citation: Suggested Citation

Ao, Rui and Xu, Xiu-Li and Zhao, Yanfang and Wang, Jun and Hu, Mei and Wang, Xiu-Juan and Boukherroub, Rabah and Chen, Xiangfeng and ZHANG, Feng, Synthesis of Cof (Tapa-Dhta)-Sodium Alginate-Ca2+-Polyacrylate Composite Microspheres as Adsorbent for Dispersive Solid Phase Extraction of Fluoroquinolone Antibiotics in Milk Prior to the Quantification by Hplc-Ms/Ms. Available at SSRN: https://ssrn.com/abstract=5217523 or http://dx.doi.org/10.2139/ssrn.5217523