Specific Enrichment of Antioxidant Peptides from Tilapia Skin Enzymatic Hydrolysate by Pmma-Mcm-41

Abstract

Peptides derived from aquatic by-products have been demonstrated to possess excellent functional properties. Methyl methacrylate (MMA), a hydrophobic monomer, was selected and successfully used to modify the mesoporous material MCM-41. The PMMA-MCM-41 material was synthesized and then applied to enrich antioxidant peptides in the enzymatic hydrolysates of tilapia skin (TSP). The adsorption behavior of PMMA-MCM-41 towards TSP has been verified to follow a pseudo-second-order adsorption kinetics model. Thermodynamic analysis reveals that the adsorption of TSP on PMMA-MCM-41 adheres more closely to the Freundlich model. PMMA-10 exhibits an adsorption capacity of 150.91 mg/g. The IC50 values for scavenging DPPH·, ABTS+·, and ·OH of the enriched peptides by PMMA-10 were 0.068±0.007, 0.178±0.005 and 0.039±0.004 mg/mL, respectively. The specific enrichment of antioxidant peptides by PMMA-10 is primarily accomplished through size-exclusion and hydrophobic interactions, thereby demonstrating the great potential of modified mesoporous materials in enriching functional peptides from aquatic products.

Keywords: MCM-41, MMA, Characterization, Adsorption behavior, Enrichment