Optimisation of Liver Homogenate Procedure to Evaluate Liver Enzymes and Lipid Biomarkers in Mice

Abstract

Liver disease accounts for approximately 2 million deaths per year worldwide, due to viral hepatitis combined with hepatocellular carcinoma. Invasive techniques like liver biopsy are a gold standard to diagnose fatty liver diseases which include various established scoring systems and indexes across the globe. Serum and liver AST and ALT levels are important biochemical indicators for hepatic cellular injury. Analysis of liver enzymes and lipids profile from serum as well as liver samples equally important for the efficacy studies which investigate the effects of drug to control the liver diseases. Various methods are available for the analysis which include different homogenate medium for enzymes and lipid profile and different reagent of commercially available kits. In the present study, we have tried to optimize the homogenization procedure by using different homogenization mediums and ratio. Again, the analysis was performed on the Rx Dytona plus instrument for both enzymes and lipids. The method was optimized with 20% PBS homogenate for liver enzymes and lipids profile on Rx Dytona plus instrument which eliminates many lab-to-lab variations.

Note:
Funding declaration: This study was conducted by Jai Research Foundation, Valvada, Gujarat, India and did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

Conflict of Interests: We wish to confirm that there are no known conflicts of interest associated with this publication, and there has been no significant financial support for this work that could have influenced its outcome.

Ethical Approval: The experimentation was approved by the “Institutional Animal Ethics Committee (IAEC)” JRF.

Keywords: Liver Homogenate, Phosphate buffer solution, Mice, Liver Enzymes, Lipid Biomarkers