A Novel Fluorescent Aptasensor Based on Boron/Nitride-Doped Carbon Nanoflower and Exo Iii-Assisted Double-Cycle Signal Amplification for the Detection of Acrylamide in Foods

Abstract

AbstractsSince acrylamide (AA) in food is a worldwide issue that endangers both human health and food safety, a highly sensitive detection technique must be developed. In this study, a novel boron nitride-doped carbon nanoflower composite (B/N-C NF) was prepared for the first time as a highly efficient quenching material. Compared with nitrogen-doped carbon material (N-C), B/N-C NF exhibits more excellent quenching performance, and the famous Stern-Volmer equation confirms that the quenching of B/N-C NF is dominated by static quenching. Three hairpin probes and Exo Ⅲ were designed to provide a double-cycle amplification system. The sensor had a linear range of 0.01-500 nM and a limit of detection of 1.802 pM. With recoveries ranging from 97.7% to 109.9%, the designed sensor were effectively employed to detect AA in spiked coffee and potato chips. The sensor was also compared with the HPLC method and both were in good agreement.

Keywords: B/N-C NF, Exo III, double-cycle amplification, FRET