Preparation of Magnetic Nanocomposite Adsorbents Using Succinic Anhydride and Maltodextrin for Solid-Phase Extraction of Four Fluoroquinolone Antibiotics and Their Determination by Hplc

Abstract

Succinic anhydride and maltodextrin (MD) as the primary components used for synthesize of the magnetic nanocomposite adsorbents. The synthesized materials were thoroughly characterized using Fourier transform infrared spectroscopy (FT-IR) to examine functional groups, scanning electron microscopy (SEM) to analyze surface morphology, and X-ray diffraction (XRD) for crystallinity and structural properties. The developed nanocomposites were subsequently applied for magnetic solid-phase extraction (MSPE) of four fluoroquinolone antibiotics (FQs), including enrofloxacin (ENR), ciprofloxacin (CIP), moxifloxacin (MOX), and ofloxacin (OFL). Extraction conditions were optimized, with the optimal parameters being 30.0 mg adsorbent amount, pH of 6.0, extraction time of 20 min, and a solution temperature of 40°C. The MSPE method was successfully employed for the extraction and preconcentration of FQs from river water samples. The extracted antibiotics were analyzed using high-performance liquid chromatography (UVHPLC), ensuring high sensitivity and selectivity. The method exhibited excellent linearity, with calibration ranges of 0.6–500.0 ng/mL for ENR and CIP, and 0.2–500.0 ng/mL for MOX and OFL. The limits of detection (LOQ) were found to be 0.2 ng/mL for ENR and CIP, and 0.05 ng/mL for MOX and OFL, while the limits of quantification (LOD) were 0.6 ng/mL and 0.2 ng/mL, respectively. These findings demonstrate the efficiency and applicability of the synthesized nanocomposites for the extraction and determination of fluoroquinolone antibiotics in environmental water samples.

Keywords: Magnetic nanocomposites, Succinic anhydride, Solid-phase extraction, Fluoroquinolone antibiotics, HPLC