Erythrocyte  Atp Levels: Impact of Low‑Level Infrared Laser Irradiation

Abstract

Background:  The low level laser therapy contributes to enhancing the various physiological effects on blood circulation. Objective: The effect of infrared laser irradiation at a wavelength of 810 nm on adenosine triphosphate levels in human red blood cells after different storage conditions was examined. Methodology: Anticoagulated blood samples from healthy volunteers were obtained and kept for testing. An infrared laser with fluencies of 30, 52, and 70 J/cm² and an output power of 10 mW for each sample was employed. Adenosine Triphosphate content in the red blood cells was quantitatively measured using Promega’s CellTiter-Glo Luminescent Assay kit. The blood was kept in storage for no more than 20 days, and the samples were taken before and after irradiation on days 0, 5, 10, 15, and 20. Results: The red blood cells count showed no statistical variances pre- and post-irradiation across all storage durations and flounces. But at a fluence of 52 J/cm², significant differences at P<0.05, P<0.05, P<0.02, and P<0.01 were recorded at 5-, 10-, 15-, and 20-day storage between the irradiated and non-irradiated red blood cells. The greatest percentage 4.1% increase in Adenosine Triphosphate was observed when the laser emissions were at 30 J/cm². Conclusions: Results provide overwhelming evidence that the samples of irradiated red blood cells at fluences of 30, 52, and 70 J/cm² demonstrated significantly higher Adenosine Triphosphate levels than the control at all-time storage intervals. This suggests that Infrared Laser irradiation may cause conformational changes on the red blood cells membrane or some of its components.

Keywords: Infrared laser, Adenosine Triphosphate level, Erythrocyte, ATP levels