Streptomyces as a Host for the Secretion of Heterologous Proteins for the Production of Biopharmaceuticals

5 Pages Posted: 3 Oct 2006

See all articles by Carlos Vallin

Carlos Vallin

Center of Pharmaceutical Chemistry, Havana, Cuba

Elsa Pimienta

Center of Pharmaceutical Chemistry, Havana, Cuba - Department of Biomedical Research

Astrid Ramos

University of California, San Francisco - Department of Biomedical Research

Caridad Rodriguez

Center of Pharmaceutical Chemistry - Department of Biomedical Research

Lieve van Mellaert

Catholic University of Leuven (KUL), Rega Institute - Laboratory of Bacteriolog

Jozef Anné

Catholic University of Leuven (KUL), Rega Institute - Laboratory of Bacteriolog

Abstract

The commercial production of therapeutic or diagnostic proteins in recombinant micro-organisms is of considerable interest. Several microbial protein production systems have been developed. So far, Escherichia coli have been the commonly employed host. However, proteins expressed in this host remain intracellular and often precipitate as inclusion bodies, which may seriously complicate downstream-processing. Faced with this problem, several genera of Gram-positive bacteria are being tested as host for the production of heterologous proteins due to their ability to efficiently secrete proteins in the culture medium. Among them is the genus Streptomyces since several of its species are known to secrete high amounts of proteins. Due to the absence of an extensive restriction-modification system, limited protease activity and the availability of suitable vector systems, Streptomyces lividans is the host of choice for the secretory production of heterologous proteins. The presented results show, that S. lividans can act as an interesting host to produce a number of proteins useful in several disease areas important in the worldwide pharmaceutical sales: i.e. oncology, immunology, cardiovascular diseases and infectious diseases.

Suggested Citation

Vallin, Carlos and Pimienta, Elsa and Ramos, Astrid and Rodriguez, Caridad and van Mellaert, Lieve and Anné, Jozef, Streptomyces as a Host for the Secretion of Heterologous Proteins for the Production of Biopharmaceuticals. Journal of Business Chemistry, Vol. 2, No. 3, pp. 107-111, September 2005. Available at SSRN: https://ssrn.com/abstract=887287

Carlos Vallin (Contact Author)

Center of Pharmaceutical Chemistry, Havana, Cuba ( email )

Havana
Cuba

Elsa Pimienta

Center of Pharmaceutical Chemistry, Havana, Cuba - Department of Biomedical Research

Havana
Cuba

Astrid Ramos

University of California, San Francisco - Department of Biomedical Research

San Francisco, CA 94143-2280
United States

Caridad Rodriguez

Center of Pharmaceutical Chemistry - Department of Biomedical Research

Havana
Cuba

Lieve Van Mellaert

Catholic University of Leuven (KUL), Rega Institute - Laboratory of Bacteriolog

Leuven
Belgium

Jozef Anné

Catholic University of Leuven (KUL), Rega Institute - Laboratory of Bacteriolog

Leuven
Belgium

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