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Metabolic Control of Gametophore Shoot Formation Through Arginine in the Moss Physcomitrella Patens
66 Pages Posted: 15 May 2020 Publication Status: Published
More...Abstract
Shoot formation is accompanied by active cell proliferation and expansion, which requires adaptation of the metabolic state to allow for developmental control. Despite the importance of such metabolic reprogramming, it remains unclear how development and metabolism are integrated. In this study, we showed that disruption of ANGUSTIFOLIA3 orthologs (PpAN3s) compromised gametophore shoot formation in the moss Physcomitrella patens due to defective cell proliferation and expansion. Trans-omics analysis revealed that the downstream activity of PpAN3 is linked to arginine metabolism. Elevating arginine level by chemical treatment led to stunted gametophores and caused Ppan3 mutant-like transcriptional changes in the wild-type plant. Furthermore, ectopic expression of AtAN3 from Arabidopsis thaliana ameliorated the defective arginine metabolism and promoted gametophore formation in Ppan3 mutants. Together, these findings indicate that arginine metabolism is a key pathway associated with gametophore formation and provide evolutionary insights into the establishment of the shoot system in land plants through the integration of developmental and metabolic processes.
Keywords: ANGUSTIFOLIA3 transcriptional co-activator, Arginine metabolism, Gametophore, Physcomitrella patens, Shoot formation, Trans-omics analysis
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