Wenfa Ng

National University of Singapore (NUS)

PhD Candidate

Singapore

SCHOLARLY PAPERS

42

DOWNLOADS

826

SSRN CITATIONS

0

CROSSREF CITATIONS

0

Scholarly Papers (42)

1.

Protocol for the Transformation of Bacillus Subtilis through Inducing Natural Competence

Number of pages: 4 Posted: 16 Apr 2021
Wenfa Ng
National University of Singapore (NUS)
Downloads 437 (95,640)

Abstract:

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Bacillus subtilis, natural competence, transformation, genetic engineering, plasmid DNA

2.

Production of Coenzyme Q10 in Recombinant Schizosaccharomyces Pombe through Metabolic Engineering Approaches

Number of pages: 19 Posted: 13 Apr 2021
Wenfa Ng
National University of Singapore (NUS)
Downloads 50 (533,403)

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metabolic engineering, flux balance, pathway analysis, metabolic flux analysis, Pathway engineering,

3.

MATLAB Software Suite That Process Raw RNA-Seq Transcriptome Dataset on a Personal Computer

Number of pages: 5 Posted: 27 Apr 2021
Wenfa Ng
National University of Singapore (NUS)
Downloads 49 (537,903)

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RNA-Seq Transcriptome, Differential Gene Expression, MATLAB Software Suite, Sequenced Read Alignment, Expression Count

4.

Understanding Spatial and Temporal Scale in Biology

Number of pages: 7 Posted: 14 Apr 2021
Wenfa Ng
National University of Singapore (NUS)
Downloads 45 (556,931)

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biological complexity, temporal scale, spatial scale, biological organization, RNA sequencing, single cell, population level, subpopulations, sampling points

5.

Possibility of teaching Bacillus subtilis ethylene glycol utilization

Number of pages: 4 Posted: 14 Apr 2021
Wenfa Ng
National University of Singapore (NUS)
Downloads 39 (587,681)

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substrate utilization, unconventional substrate, metabolic engineering, ethylene glycol, alternative feedstock

6.

Predicting Regulatory Motifs of Enzymes Using Machine Learning

Number of pages: 3 Posted: 15 Apr 2021
Wenfa Ng
National University of Singapore (NUS)
Downloads 25 (676,226)

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regulatory motifs, enzymes, computational structure prediction, protein-ligand affinity predictions,

7.

Evaluating Potential of Ethanol-Based NADH Regeneration in Supporting Whole-Cell Biocatalysis

Number of pages: 8 Posted: 16 Apr 2021
Wenfa Ng
National University of Singapore (NUS)
Downloads 24 (683,666)

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NADH regeneration, ethanol utilization pathway, glucose dehydrogenase, biotransformation reaction, biocatalysis

8.

Examining How Different Carbon Entry Point Affects Recombinant Protein Production from Ethylene Glycol in Bacillus Subtilis

Number of pages: 5 Posted: 14 Apr 2021
Wenfa Ng
National University of Singapore (NUS)
Downloads 18 (731,722)

Abstract:

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carbon entry point, substrate utilization, recombinant protein, flux distribution, amino acid synthesis

9.

Good Expression of cpsADH in Recombinant Escherichia coli Cultivated in LB and M9 Ethanol Medium

Number of pages: 5 Posted: 11 Feb 2022
Wenfa Ng
National University of Singapore (NUS)
Downloads 17 (740,323)

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Escherichia coli, cpsADH, LB medium, M9 ethanol medium, growth medium effect

10.

Phylogenetic Tree of Helicobacter Species

Number of pages: 3 Posted: 01 Dec 2021
Wenfa Ng
National University of Singapore (NUS)
Downloads 16 (749,043)

Abstract:

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Helicobacter pylori, Helicobacter nemestrinae, phylogenetic tree, 16S rRNA gene, evolutionary relationships,

11.

Inelastic Nature of Labour Market Needs a Progressive Wage Structure to Equilibrate Skills and Salary Compensation

Number of pages: 2 Posted: 22 Mar 2021
Wenfa Ng
National University of Singapore (NUS)
Downloads 14 (767,475)

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labour market, skills qualification, salary compensation, externalities, inelastic market

12.

Database of Small Proteins of Fewer Than 50 Amino Acids in Different Microbial Species

Number of pages: 3 Posted: 06 Jun 2022
Wenfa Ng
National University of Singapore (NUS)
Downloads 13 (776,987)

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small protein database, microbial species, proteome, number of residues, molecular weight

13.

Preprints as Medium for Teaching Science Writing

Number of pages: 3 Posted: 12 Nov 2020
Wenfa Ng
National University of Singapore (NUS)
Downloads 12 (786,671)

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science writing, preprints, post-publication peer review, updatable writing, critical thinking

14.

Illustrative Protocol For Determining The Centrifugation Parameters of Bacterial Cells

Number of pages: 4 Posted: 01 Aug 2022
Wenfa Ng
National University of Singapore (NUS)
Downloads 10 (806,529)

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Escherichia coli, centrifugation parameters, rotational speed, time duration, separation performance,

15.

Database of Endogenized Phage Genes of Different Microbial Species

Number of pages: 3 Posted: 22 Jul 2022
Wenfa Ng
National University of Singapore (NUS)
Downloads 9 (816,879)

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bacteriophage, lysogeny, endogenized phage gene, database, bacterial species

16.

Development of Protocol for Dry Heat Inactivation of Escherichia Coli DH5α

Number of pages: 4 Posted: 17 Feb 2022
Wenfa Ng
National University of Singapore (NUS)
Downloads 8 (827,502)

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dry heat, Escherichia coli, pore water, surface water, water of hydration

17.

Gene Expression Database of Different Microbial Species

Number of pages: 4 Posted: 01 Aug 2022
Wenfa Ng
National University of Singapore (NUS)
Downloads 7 (838,235)

Abstract:

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Gene expression, RNA-seq, DNA microarray, cell differentiation, cellular physiology

18.

Citizen Science Mini-Project: Know Your Microbe Through Its 16S rRNA Gene Sequence

Number of pages: 9 Posted: 04 Jun 2021
Wenfa Ng
National University of Singapore (NUS)
Downloads 6 (849,290)

Abstract:

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16S rRNA Gene, Microbial Identification, Phylogenetics, BLAST Search

19.

Development of a Proteome Database of Prokaryotic and Eukaryotic Microorganisms

Number of pages: 4 Posted: 08 Aug 2022
Wenfa Ng
National University of Singapore (NUS)
Downloads 5 (860,587)

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Proteome database, prokaryotes, eukaryotes, molecular cloning, protein identification, molecular weight distribution,

20.

Citizen Science Mini-Project: Learning the Structure of Scientific Names

Number of pages: 3 Posted: 05 May 2021
Wenfa Ng
National University of Singapore (NUS)
Downloads 5 (860,587)

Abstract:

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scientific name, model organism, genus, species, family name, first name

21.

Developing a Central Theme for a Tutorial Set Facilitates Learning of Concepts

Number of pages: 3 Posted: 11 Nov 2020
Wenfa Ng
National University of Singapore (NUS)
Downloads 5 (860,587)

Abstract:

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tutorial, theme, concepts, question set, framework

22.

26S rRNA Gene Is not Useful for Identifying Saccharomycodes Ludwigii by qPCR

Number of pages: 2 Posted: 14 Feb 2022
Wenfa Ng
National University of Singapore (NUS)
Downloads 4 (872,597)

Abstract:

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Saccharomycodes ludwigii, quantitative polymerase chain reaction, 26S rRNA gene, microbial identification

23.

Citizen Science Mini-Project: Understanding Shapes of Microbes Through Electron Micrographs

Number of pages: 9 Posted: 05 May 2021
Wenfa Ng
National University of Singapore (NUS)
Downloads 4 (872,597)

Abstract:

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microbe shape, microorganisms, electron micrographs, classification

24.

Annotation of the protocol for preparing chemically competent cells

Number of pages: 2
Wenfa Ng
National University of Singapore (NUS)
Downloads 2

Abstract:

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chemically competent cells, transformation protocol, annotation,

25.

Escherichia coli cells stored at 4 oC could be used as inoculum to activate a microbial culture

Number of pages: 4
Wenfa Ng
National University of Singapore (NUS)
Downloads 1

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Escherichia coli, anaerobic culture, aerobic culture, LB medium,

26.

Possible degradation of plasmid in Escherichia coli DH5α stored at 4 oC

Number of pages: 3
Wenfa Ng
National University of Singapore (NUS)
Downloads 1

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Escherichia coli, plasmid degradation, cold storage,

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currency’s price, market-based mechanism, information transmission, central bank, proper valuation

28.

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Reserve Currency, Government Bonds, Default Risk, Stability, Operational Expenditure

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currency price, terms of trade, free market mechanism, currency trading, trading position

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economic equilibrium, subsidy, bike sharing, low interest loans, social benefits

31.

State Ownership of Public Transportation Assets

Posted: 11 Nov 2020
Wenfa Ng
National University of Singapore (NUS)

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cost model, public transport, public ownership of buses and trains, government approved fare rates, improving efficiencies, legacy equipment, operating cost

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liquidity, transaction time, time variation of asset value, financial asset, property transaction

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terms of trade, price of currency, equilibrium price, supply and demand, price discovery

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currency manipulation, terms of trade, financial speculation, currency peg, foreign exchange, interest rate, open market operations, bank reserve ratio, central bank, banking system

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Public Transport, Auction Theory, Operating Cost, Transport Economics, Rail, Bus Service, Route Planning, Assets

36.

Value and Disvalue of a Strong Currency

Posted: 29 Nov 2016
Wenfa Ng
National University of Singapore (NUS)

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exchange rate, balance of payments, capital account, trade balance, trade deficits, currency trading

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central bank, finance, governance structure, interest rate, monetary policy, fiscal policy

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transfer payments, subsidies, common currency, currency union, monetary union, fiscal union, free trade area, trade liberalization, tariffs, Eurozone,

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commodity supercycle, business cycle theory, financial engineering, quantitative modeling, trade geography, international trade, offshoring, division of labor,

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shotgun metagenomics, microbial ecology, microbial identification, BLAST, read length,

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background fluorescence, quantitative polymerase chain reaction, fluorescent probe,

42.

No endogenized phage gene in Helicobacter pylori genome

Number of pages: 1
Wenfa Ng
National University of Singapore (NUS)
Downloads 0

Abstract:

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Helicobacter pylori, bacteriophage, evolutionary rate, DNA polymerase, natural selection,

Other Papers (4)

Total Downloads: 86
1.

The Value of a Honours Degree Is in Educating An Individual to Tackle Problems in Life

Number of pages: 4 Posted: 12 Apr 2021
Wenfa Ng
National University of Singapore (NUS)
Downloads 73

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honours degree, critical thinking, taught courses, hypothesis, research project, problem solving, independent inquiry, seminar teaching, primary sources, experimentation

2.

Standardized Marker Size and Line Width Helps Reduce Distorted Presentation of Graphs to Readers

Number of pages: 4 Posted: 12 Apr 2021
Wenfa Ng
National University of Singapore (NUS)
Downloads 6

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marker size, line width, distortion, graph, visual attention

3.

Citizen Science Mini-Project: Gleaning Useful Insights About a Microbe From Its Wikipedia Webpage

Number of pages: 6 Posted: 28 May 2021
Wenfa Ng
National University of Singapore (NUS)
Downloads 5

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microbe, defining characteristics, Wikipedia, workflow,

4.

Citizen Science Mini-Project: Understanding the Structural Basis of Protein Function

Number of pages: 7 Posted: 13 May 2021
Wenfa Ng
National University of Singapore (NUS)
Downloads 2

Abstract:

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protein structure, X-ray crystallography, homology modelling, protein function