header

Multifunctional Theranostic Contrast Agent for US/NIRF-Based Tumor Diagnosis and US-Triggered Combined Photothermal and Gene Therapy

34 Pages Posted: 10 May 2019 Publication Status: Accepted

See all articles by Ling Wang

Ling Wang

affiliation not provided to SSRN

Hangqing Lu

affiliation not provided to SSRN

Qi Gao

affiliation not provided to SSRN

Chenyan Yuan

affiliation not provided to SSRN

Fengan Ding

affiliation not provided to SSRN

Jia Li

affiliation not provided to SSRN

Dongsheng Zhang

affiliation not provided to SSRN

Xilong Ou

affiliation not provided to SSRN

Abstract

Purpose: Encapsulated microbubbles (MBs) have been reported asnew theranostic carriers for simultaneous imaging and ultrasound (US)-triggered therapy. Here, we designed a dual-modality US/NIRF contrastagent and extended its applications from image contrast enhancement tocombined diagnosis and therapy with US-directed and site-specifictargeting.

Methods: Gold nanorods (AuNRs) resonant at 880 nm together with theNIR797 dye were first encapsulated in lipid-shelled MBs to constructfluorescent gold microbubbles (NIR797/AuMBs) via thin film hydration andmechanical shaking in the presence of sulfur hexafluoride (SF6) gas.Then, polyethylenimine (PEI)-DNA complexes were electrostaticallyconjugated onto the surface of the NIR797/AuMBs, forming theranosticencapsulated MBs (PEI-DNA/NIR797/AuMBs). The potential of the PEIDNA/NIR797/AuMBs for use as a dual-modality contrast enhancement agentwas evaluated in vitro and in vivo. The antitumor effect of US/NIR laserirradiation mediating double-fusion suicide gene and photothermal therapywas also investigated using Bel-7402 cells and xenografts.

Results: The developed theranostic AuMB complexes could not only provideexcellent US and NIRF imaging to detect tumors but also serve as anefficient US-triggered carrier for gene delivery and photothermalablation of tumors in xenografted nude mice. And US + laser exposuregroup showed a much higher rate of cell inhibition, apoptosis andnecrosis as well as a higher Bel-7402 xenograft inhibition rate than thesingle gene therapy or single exposure (US or laser) group.

Conclusions: PEI-DNA/NIR797/AuMBs would be of great value for providingmore comprehensive diagnostic information and to guide more accurate andeffective synergistic cancer therapy.

Keywords: gold nanorods, theranostics, dual-modality imaging, microbubble, photothermal therapy

Suggested Citation

Wang, Ling and Lu, Hangqing and Gao, Qi and Yuan, Chenyan and Ding, Fengan and Li, Jia and Zhang, Dongsheng and Ou, Xilong, Multifunctional Theranostic Contrast Agent for US/NIRF-Based Tumor Diagnosis and US-Triggered Combined Photothermal and Gene Therapy (May 10, 2019). Available at SSRN: https://ssrn.com/abstract=3385801 or http://dx.doi.org/10.2139/ssrn.3385801

Ling Wang (Contact Author)

affiliation not provided to SSRN

Hangqing Lu

affiliation not provided to SSRN

No Address Available

Qi Gao

affiliation not provided to SSRN

Chenyan Yuan

affiliation not provided to SSRN

No Address Available

Fengan Ding

affiliation not provided to SSRN

No Address Available

Jia Li

affiliation not provided to SSRN

Dongsheng Zhang

affiliation not provided to SSRN

Xilong Ou

affiliation not provided to SSRN

No Address Available

Do you have a job opening that you would like to promote on SSRN?

Paper statistics

Downloads
74
Abstract Views
430
PlumX Metrics