Enhanced Methanol Tolerance of Zif-8-Immobilized Aspergillus Oryzae Lipase for Biodiesel Production from Used Cooking Oil

Abstract

The absorption process of Aspergillus oryzae lipase (AOL) onto the surface of ZIF-8 was elucidated using molecular dynamic (MD) simulation. AOL are absorped onto the surface of ZIF-8 via hydrogen bonding, electrostatic and van der Waals interaction with minimal changes to its native configurations. Unlike free AOL, AOL@ZIF-8 can maintain its structure in simulated methanol solutions. To verify the results from MD simulation, AOL was immobilized onto ZIF-8 (AOL@ZIF-8: immobilization efficiency of 64.71 % and a protein loading of 57.36 mg/g) and characterized using SEM, FTIR, and XRD. As compared to free AOL, AOL@ZIF-8 demonstrated higher transesterification activity with an activity recovery of 106.18%. In agreement with MD simulations, AOL@ZIF-8 exhibited enhanced stability in wide range of pH and under presence of alcohol solution. AOL@ZIF-8 can be used to catalyze transesterification of used cooking oil for production of biodiesel (yield of 81.19 %).

Keywords: Molecular dynamic simulation, lipase immobilization, biodiesel